MAO can catalysis 4-dimethylambenzylamine to produce p-dimethylaminobenzaldehyde. p-Dimethylaminobenzaldehyde has a characteristic absorption peak at 355 nm. The activity of MAO can be calculated indirectly by analyzing the production of p-dimethylaminobenzaldehyde.

Performance characteristics

Synonyms	MAO
Sample type	Animal tissue
Sensitivity	6 U/L
Detection range	6-722 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	80 min
Precision	Average inter-assay CV: 3.300%Average intra-assay CV: 2.700%
Other instruments required	Micropipettor, Vortex mixer, Incubator, Tubes
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (6-722 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Mouse liver tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat lung tissue homogenate	1
10% Mouse kidney tissue homogenate	1
10% Rat brain tissue homogenate	1
Human serum	1

Note: The diluent is reagent 3.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022) IF: 5.895
Screening of Monoamine Oxidase B Inhibitors from Fragaria nubicola by Ligand Fishing and Their Neuroprotective Effects

DOI: 10.1021/acs.jafc.2c06630

Sample: PC12 cells

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