Total Phenols Colorimetric Assay Kit (Plant samples)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K354-S

      Size:
      • 100 Assays
      • 200 Assays
      Qty:
      - +
      Price: $220

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      Application

      This kit can be used to measure the total phenols content in plant tissue samples.


      Detection significance

      Plant total phenol is a common secondary natural metabolite in plants. There are several kinds of phenolic compounds, such as hydroxybenzoic acid, hydroxy cinnamic acid, flavonoids, chalcone, flavonoids, lignin, coumarin and astragalus. Phenolic compounds are antioxidants that delay or prevent oxidation and oxygen radical reactions.

       

      Detection principle

      Under alkaline conditions, tungsten-molybdenum acid can be reduced by phenols and produce blue compounds, which has a characteristic absorption peak at 760 nm. The content of total phenols in sample can be calculated indirectly by measuring the absorbance at 760 nm.


      Experimental instrument

      Incubator, 40 Mesh sieve, Ultrasonic cell disruptor, Centrifuge, Spectrophotometer (760 nm)


      Pretreatment of sample

      1.   Take fresh plant tissue (5-10 g), rinse the surface with distilled water and dry with filter paper. Then dry to constant weight in a vacuum drying oven at 40 (The difference between the two weights should be less 0. 3 mg). Crush and screen with 40 mesh sieve, sealed at room temperature.

      2.    Weigh 0.1 g crushed sample and add 2.5 mL of 60% ethanol. Treat the sample with sonication (power: 300W, 3 seconds/time, interval for 4 seconds, total: 30 min). Centrifuge at 10000 g for 10 min at 25. Take the supernatant for detection.



      Operation steps

      1.    The dilution of standard

      Dilute 1 mg/mL o-dihydroxybenzene solution with double distilled water to a serial concentration. The recommended dilution gradient is as follows: 150, 120, 100, 80, 40, 20, 0 μg/mL.

      2.    Operation table

       

      Control tube

      Standard tube

      Sample tube

      Sample (mL)

      0.1

       

      0.1

      O-dihydroxybenzene with different concentration (mL)

       

      0.1

       

      Reagent 1 (mL)

       

      0.5

      0.5

      Mix fully and stand for 2 min at room temperature.

      Reagent 2 application solution (mL)

      0.5

      0.5

      0.5

      Double-distilled water (mL)

      1.4

      0.9

      0.9

      Mix fully and stand for 10 min at room temperature. Set to zero with double-distilled water and measure the absorbance values of each tube at 760 nm with 0.5 cm diameter cuvette.



      Technical parameter

      1. The sensitivity of the kit is 0.73 μg/mL.

      2. The intra-assay CV is 1.9% and the inter-assay CV is 2.5%.

      3. The recovery of the kit is 101%.

      4. The detection range of the kit is 0.73-150 μg/mL.

       

      Notes

      1. The kit is for scientific research only.

      2. Instructions should be followed strictly, changes of operation may result in unreliable results.

      3. The validity of kit is 6 months.

      4. Do not use components from different batches of kit.

      5. O-dihydroxybenzene standard solution should be prepared freshly, as it is easily oxidized.






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