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Total Phenols Colorimetric Assay Kit (Plant samples)

Cat.No.:E-BC-K354-M
Detection instrument: Microplate reader(750 nm-770 nm,optimum wavelength: 760 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Plant total phenol is a common secondary natural metabolite in plants. There are several kinds of phenolic compounds, such as hydroxybenzoic acid, hydroxy cinnamic acid, flavonoids, chalcone, flavonoids, lignin, coumarin and astragalus. Phenolic compounds are antioxidants that delay or prevent oxidation and oxygen radical reactions.

Performance characteristics

Sample type	Plant tissue
Sensitivity	1.05 μg/mL
Detection range	1.05–148 μg/mL
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	35 min
Precision	Average inter-assay CV: 4.400%Average intra-assay CV: 4.100%
Other instruments required	Incubator, Vacuum drying oven, Centrifuge
Other reagents required	60% alcohol
Storage	2-8℃
Valid period	12 months

Images

The concentration of total phenol was determined.

JH Kim et al investigate the bisphenol A-induced inflammation and cytotoxicity blocked by Grifola frondosa extract. Total phenol level in plant tissue was determined using total ohenol colorimetric assay kit (E-BC-K354-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.05-148 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Epipremnum aureum tissue homogenate	20-30
Daucus carota tissue homogenate	5-15
Spinacia oleracea tissue homogenate	15-25
Leek tissue homogenate	10-20

Note: The diluent is 60% alcohol.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Nutrients (2022) IF: 6.706
Grifola frondosa Extract Containing Bioactive Components Blocks Skin Fibroblastic Inflammation and Cytotoxicity Caused by Endocrine Disrupting Chemical, Bisphenol A

DOI: 10.3390/nu14183812

PMID: 36145189
Sample: fruit

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