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Biuret Protein Colorimetric Assay Kit

Cat.No.:E-BC-K165-S
Detection instrument: Spectrophotometer(540 nm)

Detection method: Colorimetric method

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Detection principle

Any compound that contains two -CONH2 in the molecule can react with alkaline copper solution to form a purple complex, which is known as the biuret reaction. Many peptide bonds (-CONH-) in protein molecules can perform this reaction, and the color degree of all kinds of proteins are essentially the same.

Performance characteristics

Synonyms	TP
Sample type	Serum,plasma,tissue
Sensitivity	0.373 g/L
Detection range	0.373-80 g/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 2.600%Average intra-assay CV: 1.200%
Other instruments required	Micropipettor, Incubator
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 3: -20℃, others: 2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.373-80 g/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Porcine serum	2-4
Human serum	2-4
5% Mouse liver tissue homogenization	1
10% Epipremnum aureum tissue homogenization	1
5% Mouse heart tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

JOURNAL OF CHEMICAL NEUROANATOMY (2020) IF: 3.052
The petrosal vein mutilation affects the SOD activity, MDA levels and AQP4 level in cerebellum and brain stem in rabbit

DOI: 10.1016/j.jchemneu.2020.101791

PMID: 32339652
Sample: Tissue homogenate
Journal of Oleo Science (2022) IF: 1.628
Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress

DOI: 10.5650/jos.ess22033

PMID: 35965085
Sample: serum
ANDROLOGIA (2020) IF: 1.951
Androgenic effect of aqueous leaf extract of Moringa oleifera on Leydig TM3 cells in vitro

DOI: 10.1111/and.13825

PMID: 32926461
Sample: Cell lysate

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