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Total Sulfhydryl Group/Total Thiol (-SH) Colorimetric Assay Kit

Cat.No.:E-BC-K265-M
Detection instrument: Microplate reader(410 nm-420 nm,optimum wavelength: 412 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly.

Performance characteristics

Synonyms	T-SH
Sample type	Serum, plasma, animal tissue
Sensitivity	9.91 μmol/L
Detection range	9.91-1000 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 2.900%Average intra-assay CV: 2.500%
Other instruments required	Micropipettor, Water bath, Incubator, Vortex mixer, Centrifuge
Other reagents required	Absolute ethyl alcohol
Storage	2-8℃
Valid period	12 months

Images

The level of total thiol was significantly changed by different type of treatment. (**P<0.01 vs. control, #P<0.05, ###P<0.001 vs. reperfusion injury)

O A Afolabi et al evaluate the testicular torsion management with multiple method of treatment. Total sulfhydryl group (-SH) level in rat testicular tissue was determined using thiol colorimetric assay kit (E-BC-K265-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (9.91-1000 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human plasma	4-6
Human serum	4-6
Human urine	1
10% Rat liver tissue homogenate	4-6
Rabbit serum	3-5
Porcine serum	4-6
10% Rat kidney tissue homogenate	4-6
10% Rat spleen tissue homogenate	4-6

Note: The diluent is normal saline (0.9% NaCl).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

DOI: 10.1016/j.sjbs.2022.103495

Sample: testicular tissue

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