Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K019-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $280
      Can detect 50 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(550 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Detection principle

      The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.

      Performance characteristics

      Synonyms T-SOD
      Sample type Serum,plasma,urine,cells,cell culture supernatant,tissue
      Sensitivity 4.7 U/mL
      Detection range 4.7-166 U/mL
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 100 min
      Precision Average inter-assay CV: 6.3%Average intra-assay CV: 2.8%
      Other instruments required Micropipettor, Vortex mixer, Water bath, Centrifuge
      Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
      Storage Reagent 4: -20℃, others: 2-8℃
      Valid period 6 months

      Dilution of sample

      The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

      Citations

      1. Antihypernociceptive and neuroprotective effects of Combretin A and Combretin B on streptozotocin-induced diabetic neuropathy in mice
        IF: 2.058
        Journal: Naunyn-Schmiedeberg's Archives of Pharmacology(2019)
        DOI: 10.1007/s00210-019-01626-1
        PMID: 30759263
        Products: E-BC-K025-S E-BC-K035-S E-BC-K019-S E-BC-K031-S 
        Sample type: Serum,Tissue homegenate
        Reactivity: Mouse
      2. Assessment of the Antioxidant Potential of Hypoestes rosea Leaf in Lead-acetate-induced Albino Rats
        Journal: Journal of Complementary and Alternative Medical Research(2020)
        DOI: 10.9734/jocamr/2020/v9i130135
        PMID: 99999999
        Products: E-BC-K019-S E-BC-K136-S 
        Sample type: Plasma
        Reactivity: Rat
      3. Assessment of the Antioxidant Potential of Hypoestes rosea Leafin Streptozotocin-induced Diabetic Albino Rats
        Journal: Journal of Complementary and Alternative Medical Research(2020)
        DOI: 10.9734/jocamr/2020/v9i430149
        PMID: 99999999
        Products: E-BC-K019-S E-BC-K136-S 
        Sample type: Serum
        Reactivity: Rat
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      • Reviews (3)
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      Verified Customer

      M*sSubmitted [ Jun 12 2019 ]

      • Application:Biochemical
      • Description:The T-SOD activity in HepG2 cell supernatant was detected by the SOD kit, By asking the loading quantity of technical support samples, they gave a reference loading quantity and the loading amount of the formal experiment is similar to that of the reference. At this point, it\'s a trusty assay kit
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      Verified Customer

      M**kSubmitted [ May 29 2019 ]

      • Application:Biochemical
      • Description:The instructions tell us the treatment method of samples in detail,But he preparation part of this biochemical Assay kit is a little too much.
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      Verified Customer

      r****eSubmitted [ May 29 2019 ]

      • Application:Biochemical
      • Description:I have consulted many literatures and know that the results of SOD activity detection by different methods will be different, so it is more difficult to find the literature of SOD activity range. I think the best part of this kit is the ‘Reference values for samples’ section in the instruction, which gives the reference range of SOD activity of various samples. Using this kit (E-BC-K019) to detect human plasma samples, the results are within the reference range provided by the kit, which saves me a lot of work.
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