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Cat.No.:E-BC-K019-S
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Synonyms | T-SOD |
Sample type | Serum,plasma,urine,cells,cell culture supernatant,tissue |
Sensitivity | 4.7 U/mL |
Detection range | 4.7-166 U/mL |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 100 min |
Precision | Average inter-assay CV: 6.300%Average intra-assay CV: 2.800% |
Other instruments required | Micropipettor, Vortex mixer, Water bath, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
Storage | Reagent 4: -20℃, others: 2-8℃ |
Valid period | 12 months |
The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
The volume of sample |
HepG2 supernatant |
1 |
50 μL |
HepG2 cell |
8-10 |
25 μL |
Mouse serum |
3-5 |
20 μL |
10% Mouse liver tissue homogenate |
40-60 |
20 μL |
10% Rat kidney tissue homogenate |
15-20 |
20 μL |
Human urine |
1 |
25 μL |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
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Sample: colon tissue |
PMID: 34482083 |
Sample: Kidney tissue |
DOI: 10.1111/pce.14263 |
|
PMID: 35040151 |
Sample: leaf |
DOI: 10.1155/2022/2232365 |
|
PMID: 35898617 |
Sample: HUVECs |
PMID: 36358477 |
Sample: liver,serum |
Sample: brain |
Sample: kidney |
PMID: 35032726 |
Sample: testis |
Sample: heart |
PMID: 36280221 |
Sample: skin tissues |
PMID: 34891114 |
Sample: serum |
DOI: 10.1155/2022/7655142 |
|
Sample: nucleus pulposus(NP) cell |
PMID: 36037870 |
Sample: serum |
Sample: serum,ruminal fluid |
PMID: 34919900 |
Sample: livers |
DOI: 10.1039/D1FO01008E |
|
PMID: 34128029 |
Sample: small intestinal |
PMID: 36375957 |
Sample: larvae |
PMID: 35933747 |
Sample: intestinal tissue,serum |
DOI: 10.1093/jac/dkac093 |
|
Sample: serum |
DOI: 10.1002/lt.26421 |
|
PMID: 35100468 |
Sample: liver |
Sample: testicular tissue |
Sample: heart tissue |
DOI: 10.1002/tox.23639 |
|
Sample: serum,NR8383 cell |
PMID: 35633452 |
Sample: plasma |
DOI: 10.1111/jdi.13690 |
|
Sample: skeletal muscle,heart,pancreas |
Sample: colon tissue |
PMID: 35181488 |
DOI: 10.2147/IJN.S159789 |
|
PMID: 29695905 |
Sample: Tissue homogenate |
PMID: 35729338 |
Sample: leaf |
PMID: 35622166 |
Sample: cheek,liver,kidney |
Sample: liver,kidney,lungs |
PMID: 36235007 |
Sample: plasma |
PMID: 35344845 |
Sample: RAW264.7 cell |
DOI: 10.3390/ani13030363 |
|
Sample: Plasma |
Sample: liver |
DOI: 10.1155/2022/9943598 |
|
PMID: 35818443 |
Sample: hippocampal tissue |
Sample: BEAS-2B cells |
Sample: serum |
DOI: 10.3390/pr10101985 |
|
Sample: gastric tissue |
DOI: 10.3390/pr10071314 |
|
Sample: stomach |
Sample: Tissue homogenate |
Sample: intestine,brain |
PMID: 35635784 |
Sample: brain tissue |
PMID: 35751788 |
Sample: hippocampal neurons |
PMID: 32220623 |
Sample: Tissue homogenate |
PMID: 33383446 |
Sample: lung tissue |
PMID: 33370579 |
Sample: SK-N-SH cell,IMR-32 cell |
DOI: 10.2147/IJGM.S367125 |
|
Sample: serum |
DOI: 10.21037/tau-22-505 |
|
Sample: kidney tissue |
DOI: 10.1155/2021/2073296 |
|
Sample: BEAS-2B cell |
Sample: hippocampal tissue |
PMID: 36252429 |
Sample: SK-N-SH cells |
Sample: serum |
Sample: Tissue homogenate(Jejunum) |
DOI: 10.1111/jfbc.14058 |
|
PMID: 34981526 |
Sample: serum |
DOI: 10.1111/jfbc.14129 |
|
PMID: 35298033 |
Sample: gastric,jejunal, colonic |
DOI: 10.1111/and.14028 |
|
PMID: 33650701 |
Sample: Tissue homogenate(testicular) |
PMID: 33830385 |
Sample: Tissue homogenate |
DOI: 10.1155/2018/7341242 |
|
Sample: kidney tissues |
DOI: 10.3390/cimb45020057 |
|
Sample: colonic tissue |
Sample: serum,liver,skin |
PMID: 29250160 |
Sample: Urine |
DOI: 10.3233/WOR-203302 |
|
PMID: 33104053 |
Sample: serum |
Sample: brain |
Sample: maize roots |
Sample: Serum |
PMID: 31346790 |
Sample: Blood,Urine |
DOI: 10.1155/2020/1807126 |
|
Sample: C2C12 cell |
Sample: serum,sciatic nerves |
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r****eSubmitted [ May 29 2019 ]
M*****iSubmitted [ Oct 09 2022 ]
Asked: Can I still use it on samples that have been treated with RIPA?
Reply
adminSubmitted [ Oct 09 2022 ]
Answered: It is usually not recommended to treat the sample with lysate. If the sample is already prepared, you'd better make a pre-test first.
M*****iSubmitted [ Aug 17 2022 ]
Asked: What is the recommended dilution factor of mouse serum?
Reply
adminSubmitted [ Aug 17 2022 ]
Answered: The reference dilution for mouse serum is 3-5 times.