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Current Position:Home>>Kits>>Metabolism Assays>> Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

Citations(71)

  • Cat.No.:E-BC-K019-S

  • Detection instrument: Spectrophotometer(550 nm)

Manual MSDS

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Detection principle

The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.

Performance characteristics

Synonyms T-SOD
Sample type Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity 4.7 U/mL
Detection range 4.7-166 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 100 min
Precision Average inter-assay CV: 6.300%Average intra-assay CV: 2.800%
Other instruments required Micropipettor, Vortex mixer, Water bath, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage Reagent 4: -20℃, others: 2-8℃
Valid period 12 months

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

The volume of sample

HepG2 supernatant

1

50 μL

HepG2 cell

8-10

25 μL

Mouse serum

3-5

20 μL

10% Mouse liver tissue homogenate

40-60

20 μL

10% Rat kidney tissue homogenate

15-20

20 μL

Human urine

1

25 μL

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Citations

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    DOI: 10.3390/antiox11112105

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  7. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
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  18. Food & Function (2021) IF: 5.396
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    DOI: 10.1039/D1FO01008E

    PMID: 34128029

    		 Sample: small intestinal
  19. Journal of Environmental Sciences (2022) IF: 5.565
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  20. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
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    DOI: 10.1016/j.intimp.2022.109114

    PMID: 35933747

    		 Sample: intestinal tissue,serum
  21. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY (2022) IF: 5.79
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    DOI: 10.1093/jac/dkac093
    Sample: serum
  22. LIVER TRANSPLANTATION (2022) IF: 5.799
    Cerium oxide nanoparticles administration during machine perfusion of discarded human livers: a pilot study

    DOI: 10.1002/lt.26421

    PMID: 35100468

    		 Sample: liver
  23. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
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    DOI: 10.1016/j.sjbs.2022.103495
    Sample: testicular tissue
  24. BIOLOGICAL TRACE ELEMENT RESEARCH (2022) IF: 4.081
    Selenium Supplementation Protects Against Arsenic-Trioxide-Induced Cardiotoxicity Via Reducing Oxidative Stress and Inflammation Through Increasing NAD+ Pool

    DOI: 10.1007/s12011-022-03478-y
    Sample: heart tissue
  25. ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.109
    hUC-MSCs exosomal miR-451 alleviated acute lung injury by modulating macrophage M2 polarization via regulating MIF-PI3K-AKT signaling pathway

    DOI: 10.1002/tox.23639
    Sample: serum,NR8383 cell
  26. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022) IF: 4.223
    Prenatal exposure to environmental pro-oxidants induces mitochondria-mediated epigenetic changes: a cross-sectional pilot study

    DOI: 10.1007/s11356-022-21059-3

    PMID: 35633452

    		 Sample: plasma
  27. Journal of Diabetes Investigation (2021) IF: 4.232
    Diet-induced prediabetes: Effects on the activity of the renin-angiotensin-aldosterone system (RAAS) in selected organs

    DOI: 10.1111/jdi.13690
    Sample: skeletal muscle,heart,pancreas
  28. Foods (2022) IF: 4.35
    Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota

    DOI: 10.3390/foods11111551
    Sample: colon tissue
  29. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    In vitro antiaging activity of polyphenol rich Polyalthia longifolia (Annonaceae) leaf extract in Saccharomyces cerevisiae BY611 yeast cells

    DOI: 10.1016/j.jep.2022.115110

    PMID: 35181488
  30. International Journal of Nanomedicine (2018) IF: 4.37
    Tanshinol borneol ester on nanostructured lipid carriers has longer brain and systemic effector retention and better antioxidant activity in vivo

    DOI: 10.2147/IJN.S159789

    PMID: 29695905

    		 Sample: Tissue homogenate
  31. Scientific Reports (2022) IF: 4.38
    Molecular mechanisms associated with microbial biostimulant-mediated growth enhancement, priming and drought stress tolerance in maize plants

    DOI: 10.1038/s41598-022-14570-7

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    		 Sample: leaf
  32. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY (2022) IF: 4.553
    Investigation of the protective effect of gel incorporating Eugenia jambolana leaf extract on 5-fluorouracil-induced oral mucositis: an animal study

    DOI: 10.1007/s00432-022-04065-3

    PMID: 35622166

    		 Sample: cheek,liver,kidney
  33. PROCESS BIOCHEMISTRY (2022) IF: 4.885
    Vernodalin regulated the NF-κβp65 signaling in inflammation of lipopolysaccharide -induced sepsis rats

    DOI: 10.1016/j.procbio.2022.10.004
    Sample: liver,kidney,lungs
  34. MOLECULES (2022) IF: 4.927
    Chemical Profile of Cyperus laevigatus and Its Protective Effects against Thioacetamide-Induced Hepatorenal Toxicity in Rats

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    PMID: 36235007

    		 Sample: plasma
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    DOI: 10.1016/j.imbio.2022.152207

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    		 Sample: RAW264.7 cell
  36. Animals (2023) IF: 3.231
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    DOI: 10.3390/ani13030363
    Sample: Plasma
  37. Environmental Research Communications (2022) IF: 3.237
    Metal accumulation in muscle and oxidative stress response in the liver of juvenile Oreochromis niloticus from contaminated sediment under a simulation of increasing temperature

    DOI: 10.1088/2515-7620/ac803d
    Sample: liver
  38. Biomed Research International (2022) IF: 3.246
    Red Raspberry Extract Decreases Depression-Like Behavior in Rats by Modulating Neuroinflammation and Oxidative Stress

    DOI: 10.1155/2022/9943598

    PMID: 35818443

    		 Sample: hippocampal tissue
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    Sample: BEAS-2B cells
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    DOI: 10.1016/j.anifeedsci.2022.115543
    Sample: serum
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    DOI: 10.3390/pr10101985
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    DOI: 10.3390/pr10071314
    Sample: stomach
  43. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY (2019) IF: 3.377
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    DOI: 10.1007/s00424-019-02311-1
    Sample: Tissue homogenate
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    DOI: 10.3390/ijerph18189449
    Sample: intestine,brain
  45. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
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    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    		 Sample: brain tissue
  46. METABOLIC BRAIN DISEASE (2022) IF: 3.584
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    DOI: 10.1007/s11011-022-01013-5

    PMID: 35751788

    		 Sample: hippocampal neurons
  47. LIFE SCIENCES (2020) IF: 3.647
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    DOI: 10.1016/j.lfs.2020.117584

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    		 Sample: Tissue homogenate
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    DOI: 10.1016/j.intimp.2020.107290

    PMID: 33383446

    		 Sample: lung tissue
  49. CELLULAR SIGNALLING (2020) IF: 3.968
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    DOI: 10.1016/j.cellsig.2020.109901

    PMID: 33370579

    		 Sample: SK-N-SH cell,IMR-32 cell
  50. International Journal of General Medicine (2022) IF: 2.145
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    DOI: 10.2147/IJGM.S367125
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    DOI: 10.21037/tau-22-505
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    DOI: 10.1155/2021/2073296
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    DOI: 10.1016/j.jstrokecerebrovasdis.2022.106817

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    DOI: 10.1093/toxres/tfac080
    Sample: serum
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    Sample: Tissue homogenate(Jejunum)
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    DOI: 10.1111/jfbc.14058

    PMID: 34981526

    		 Sample: serum
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    DOI: 10.1111/jfbc.14129

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    		 Sample: gastric,jejunal, colonic
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    DOI: 10.1111/and.14028

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    		 Sample: Tissue homogenate(testicular)
  60. ECOTOXICOLOGY (2021) IF: 2.823
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    		 Sample: Tissue homogenate
  61. Journal of Diabetes Research (2018) IF: 2.885
    The Effects of Momordica balsamina Methanolic Extract on Kidney Function in STZ-Induced Diabetic Rats: Effects on Selected Metabolic Markers

    DOI: 10.1155/2018/7341242
    Sample: kidney tissues
  62. CURRENT ISSUES IN MOLECULAR BIOLOGY (2023) IF: 2.976
    Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling Pathways

    DOI: 10.3390/cimb45020057
    Sample: colonic tissue
  63. BIOLOGICAL RHYTHM RESEARCH (2022) IF: 1.219
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    DOI: 10.1080/09291016.2022.2043588
    Sample: serum,liver,skin
  64. Experimental and Therapeutic Medicine (2018) IF: 1.41
    Rutin inhibits coronary heart disease through ERK1/2 and Akt signaling in a porcine model

    DOI: 10.3892/etm.2017.5365

    PMID: 29250160

    		 Sample: Urine
  65. WORK-A Journal of Prevention Assessment & Rehabilitation (2021) IF: 1.505
    Evaluation of oxidative stress and pro-inflammatory cytokines in occupationally cadmium exposed workers

    DOI: 10.3233/WOR-203302

    PMID: 33104053

    		 Sample: serum
  66. BRAZILIAN JOURNAL OF BIOLOGY (2023) IF: 1.651
    Neuroprotective potential of lignan-rich fraction of Piper cubeba L. by improving antioxidant capacity in the rat's brain

    DOI: 10.1590/1519-6984.266573
    Sample: brain
  67. Journal of Plant Interactions (2018) IF: 1.655
    Brevibacillus sp. promotes maize root colonization by Acaulospora tuberculata and the alteration of associated plant protein responses

    DOI: 10.1080/17429145.2018.1547844
    Sample: maize roots
  68. Molecular Medicine Reports (2018) IF: 1.922
    Isopsoralen regulates PPAR‑γ/WNT to inhibit oxidative stress in osteoporosis

    DOI: 10.3892/mmr.2017.7954
    Sample: Serum
  69. ENVIRONMENTAL MONITORING AND ASSESSMENT (2019) IF: 1.959
    Biochemical investigation of association of arsenic exposure with risk factors of diabetes mellitus in Pakistani population and its validation in animal model

    DOI: 10.1007/s10661-019-7670-2

    PMID: 31346790

    		 Sample: Blood,Urine
  70. Journal of Toxicology (2020)
    Styrene Oxide Caused Cell Cycle Arrest and Abolished Myogenic Differentiation of C2C12 Myoblasts

    DOI: 10.1155/2020/1807126
    Sample: C2C12 cell
  71. Advances in Traditional Medicine (2020)
    Anti-hypernociceptive effects of methanol extract of Boswellia dalzielii on STZ-induced diabetic neuropathic pain

    DOI: 10.1007/s13596-019-00411-y
    Sample: serum,sciatic nerves
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A

Verified Customer

M*sSubmitted [ Jun 12 2019 ]

  • Application:Biochemical
  • Description:The T-SOD activity in HepG2 cell supernatant was detected by the SOD kit, By asking the loading quantity of technical support samples, they gave a reference loading quantity and the loading amount of the formal experiment is similar to that of the reference. At this point, it\'s a trusty assay kit
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Verified Customer

M**kSubmitted [ May 29 2019 ]

  • Application:Biochemical
  • Description:The instructions tell us the treatment method of samples in detail,But he preparation part of this biochemical Assay kit is a little too much.
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Verified Customer

r****eSubmitted [ May 29 2019 ]

  • Application:Biochemical
  • Description:I have consulted many literatures and know that the results of SOD activity detection by different methods will be different, so it is more difficult to find the literature of SOD activity range. I think the best part of this kit is the ‘Reference values for samples’ section in the instruction, which gives the reference range of SOD activity of various samples. Using this kit (E-BC-K019) to detect human plasma samples, the results are within the reference range provided by the kit, which saves me a lot of work.
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Q

M*****iSubmitted [ Oct 09 2022 ]

Asked: Can I still use it on samples that have been treated with RIPA?

Reply

A

adminSubmitted [ Oct 09 2022 ]

Answered: It is usually not recommended to treat the sample with lysate. If the sample is already prepared, you'd better make a pre-test first.

Q

M*****iSubmitted [ Aug 17 2022 ]

Asked: What is the recommended dilution factor of mouse serum?

Reply

A

adminSubmitted [ Aug 17 2022 ]

Answered: The reference dilution for mouse serum is 3-5 times.

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