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Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)

Citations(3)Uniprot : ml,yhyj
  • Cat.No.:E-BC-K019-S

  • Detection instrument: Spectrophotometer(550 nm)

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Detection principle

The superoxide anion free radical (O2-) can be produced by xanthine and xanthine oxidase reaction system, O2- oxidize hydroxylamine to form nitrite, it turn to purple under the reaction of developer. When the measured samples containing SOD, the SOD can specifically inhibit superoxide anion free radical (O2-). The inhibitory effect of SOD can reduce the formation of nitrite, the absorbance value of sample tube is lower than control tube. Calculate the SOD of sample according to the computational formula.

Performance characteristics

Synonyms T-SOD
Sample type Serum,plasma,urine,cells,cell culture supernatant,tissue
Sensitivity 4.7 U/mL
Detection range 4.7-166 U/mL
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 100 min
Precision Average inter-assay CV: 6.3%Average intra-assay CV: 2.8%
Other instruments required Micropipettor, Vortex mixer, Water bath, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)
Storage Reagent 4: -20℃, others: 2-8℃
Valid period 6 months

Dilution of sample

The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

The volume of sample

HepG2 supernatant

1

50 μL

HepG2 cell

8-10

25 μL

Mouse serum

3-5

20 μL

10% Mouse liver tissue homogenate

40-60

20 μL

10% Rat kidney tissue homogenate

15-20

20 μL

Human urine

1

25 μL

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Naunyn-Schmiedeberg's Archives of Pharmacology (2019) IF: 2.058
    Antihypernociceptive and neuroprotective effects of Combretin A and Combretin B on streptozotocin-induced diabetic neuropathy in mice

    DOI: 10.1007/s00210-019-01626-1

    PMID: 30759263

    Sample: Serum,Tissue homegenate
  2. Journal of Complementary and Alternative Medical Research (2020)
    Assessment of the Antioxidant Potential of Hypoestes rosea Leaf in Lead-acetate-induced Albino Rats

    DOI: 10.9734/jocamr/2020/v9i130135

    PMID: 99999999

    Sample: Plasma
  3. Journal of Complementary and Alternative Medical Research (2020)
    Assessment of the Antioxidant Potential of Hypoestes rosea Leafin Streptozotocin-induced Diabetic Albino Rats

    DOI: 10.9734/jocamr/2020/v9i430149

    PMID: 99999999

    Sample: Serum
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Reviews/Q&A

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Verified Customer

M*sSubmitted [ Jun 12 2019 ]

  • Application:Biochemical
  • Description:The T-SOD activity in HepG2 cell supernatant was detected by the SOD kit, By asking the loading quantity of technical support samples, they gave a reference loading quantity and the loading amount of the formal experiment is similar to that of the reference. At this point, it\'s a trusty assay kit
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Verified Customer

M**kSubmitted [ May 29 2019 ]

  • Application:Biochemical
  • Description:The instructions tell us the treatment method of samples in detail,But he preparation part of this biochemical Assay kit is a little too much.
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Verified Customer

r****eSubmitted [ May 29 2019 ]

  • Application:Biochemical
  • Description:I have consulted many literatures and know that the results of SOD activity detection by different methods will be different, so it is more difficult to find the literature of SOD activity range. I think the best part of this kit is the ‘Reference values for samples’ section in the instruction, which gives the reference range of SOD activity of various samples. Using this kit (E-BC-K019) to detect human plasma samples, the results are within the reference range provided by the kit, which saves me a lot of work.
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