Detection method: Colorimetric method
Detection instrument: Spectrophotometer(550 nm)
|Sample type||Serum,plasma,urine,cells,cell culture supernatant,tissue|
|Detection range||4.7-166 U/mL|
|Detection method||Colorimetric method|
|Assay type||Enzyme Activity|
|Assay time||100 min|
|Precision||Average inter-assay CV: 6.3%Average intra-assay CV: 2.8%|
|Other instruments required||Micropipettor, Vortex mixer, Water bath, Centrifuge|
|Other reagents required||Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4)|
|Storage||Reagent 4: -20℃, others: 2-8℃|
|Valid period||6 months|
The optimal sampling volume are different for different species, the SOD also are different for different samples. It is recommended to take 2~3 samples to do a pre-experiment, diluting a series of diluent and determine the dilution factor when the SOD inhibition ratio is 15%~55% (the optimal inhibition ratio is the range of 25%~45%.) before formal experiment.
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).