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Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)

Citations(51)

  • Cat.No.:E-BC-K238

  • Detection instrument: Microplate reader(510 nm), Biochemistry analyzer(510 nm)

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Detection principle

The color depth of the generated quinones is directly proportional to the triglyceride content. The absorbance values of the standard tube and the sample tube are measured respectively, and the triglyceride content in the sample can be calculated.

Performance characteristics

Synonyms TG
Sample type Serum,plasma,cells,culture supernatant
Detection range 0-9.04 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 20 min
Storage 2-8℃
Valid period 12 months

Images

Plasma TG level shows no significant differences with different diets (P<0.05).

N A M Omar et al investigate the effect of high intakes of fructose and galactose, with or without added fructooligosaccharides (FOS), on metabolism factors, inflammation, and gut integrity markers in rats. Triglyceride (TG) level in rat plasma was determined using TG colorimetric assay kit (E-BC-K238).

Citations

  1. Nature Communications (2022) IF: 17.694
    Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease

    DOI: 10.1038/s41467-022-35481-1

    PMID: 36572670

    		 Sample: liver tissue
  2. BIOMATERIALS (2022) IF: 15.304
    Self-amplified ROS production from fatty acid oxidation enhanced tumor immunotherapy by atorvastatin/PD-L1 siRNA lipopeptide nanoplexes

    DOI: 10.1016/j.biomaterials.2022.121902
    Sample: B16-F10 cell,CT-26 cell
  3. Gut Microbes (2021) IF: 10.245
    Bacteroides uniformis combined with fiber amplifies metabolic and immune benefits in obese mice

    DOI: 10.1080/19490976.2020.1865706

    PMID: 33499721

    		 Sample: Tissue homogenate
  4. SCIENCE OF THE TOTAL ENVIRONMENT (2022) IF: 10.753
    Combined effect of polystyrene microplastics and bisphenol A on the human embryonic stem cells-derived liver organoids: The hepatotoxicity and lipid accumulation

    DOI: 10.1016/j.scitotenv.2022.158585

    PMID: 36089014

    		 Sample: liver organoids (LOs)
  5. BMC BIOLOGY (2022) IF: 7.431
    Histone acetyltransferase NAA40 modulates acetyl-CoA levels and lipid synthesis

    DOI: 10.1186/s12915-021-01225-8

    PMID: 35057804

    		 Sample: L3 larvae
  6. Frontiers in Endocrinology (2022) IF: 6.055
    Total Flavonoids of Drynariae Rhizoma Improve Glucocorticoid-Induced Osteoporosis of Rats: UHPLC-MS-Based Qualitative Analysis, Network Pharmacology Strategy and Pharmacodynamic Validation

    DOI: 10.3389/fendo.2022.920931
    Sample: Serum
  7. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Peroxisome Proliferator-Activated Receptor-α (PPARα) Expression in a Clinical Population of Pakistani Patients with Type 2 Diabetes and Dyslipidemia

    DOI: 10.3390/ijms231810847
    Sample: blood
  8. Food & Function (2023) IF: 6.317
    Analysis of the mechanism of action of quercetin in the treatment of hyperlipidemia based on metabolomics and intestinal flora

    DOI: 10.1039/D2FO03509J
    Sample: serum
  9. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2021) IF: 6.514
    Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms

    DOI: 10.1016/j.ejmech.2021.113388

    PMID: 33784603

    		 Sample: serum
  10. Frontiers in Cell and Developmental Biology (2021) IF: 6.684
    N6-Methyladenosine Methyltransferase METTL3 Promotes Angiogenesis and Atherosclerosis by Upregulating the JAK2/STAT3 Pathway via m6A Reader IGF2BP1.

    DOI: 10.3389/fcell.2021.731810
    Sample: plasma
  11. Nutrients (2022) IF: 6.706
    Ascophyllum nodosum and Fucus vesiculosus Extracts Improved Lipid Metabolism and Inflammation in High-Energy Diet–Induced Hyperlipidemia Rats

    DOI: 10.3390/nu14214665
    Sample: liver
  12. LIFE SCIENCES (2021) IF: 5.037
    Pancreastatin inhibitor PSTi8 prevents free fatty acid-induced oxidative stress and insulin resistance by modulating JNK pathway: In vitro and in vivo findings

    DOI: 10.1016/j.lfs.2021.120221

    PMID: 34902437

    		 Sample: plasma
  13. FASEB JOURNAL (2021) IF: 5.192
    Holdemanella biformis improves glucose tolerance and regulates GLP-1 signaling in obese mice

    DOI: 10.1096/fj.202100126R

    PMID: 34143451

    		 Sample: blood
  14. EUROPEAN JOURNAL OF PHARMACOLOGY (2023) IF: 5.195
    Pancreastatin inhibitor PSTi8 ameliorates streptozotocin-induced diabetes by suppressing hepatic glucose production

    DOI: 10.1016/j.ejphar.2023.175559
    Sample: plasma
  15. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Integrating metabolomics and network pharmacology to investigate Panax japonicus prevents kidney injury in HFD/STZ-induced diabetic mice

    DOI: 10.1016/j.jep.2022.115893
    Sample: serum
  16. EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 5.195
    Anti-pancreatic lipase and anti-adipogenic effects of 5, 7, 3′,4′,5’ -pentamethoxy and 6, 2′,4′-trimethoxy flavone - An In vitro study

    DOI: 10.1016/j.ejphar.2022.175445
    Sample: 3T3-L1 adipocytes
  17. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079
    Sample: serum
  18. ENVIRONMENTAL MICROBIOLOGY (2022) IF: 5.491
    An inactivating mutation in the vacuolar arginine exporter gene Vae results in culture degeneration in the fungus Metarhizium robertsii

    DOI: 10.1111/1462-2920.15982

    PMID: 35352870
  19. Frontiers in Endocrinology (2021) IF: 5.555
    Metabolic Consequences of Neuronal HIF1α-Deficiency in Mediobasal Hypothalamus in Mice

    DOI: 10.3389/fendo.2021.668193

    PMID: 34733235

    		 Sample: serum
  20. Nutrients (2022) IF: 5.719
    The Allium Derivate Propyl Propane Thiosulfinate Exerts Anti-Obesogenic Effects in a Murine Model of Diet-Induced Obesity

    DOI: 10.3390/nu14030440

    PMID: 35276798

    		 Sample: plasma
  21. ACS Omega (2022) IF: 4.132
    Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion

    DOI: 10.1021/acsomega.2c03619

    PMID: 35967043

    		 Sample: serum
  22. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021) IF: 4.223
    Biochemical investigation of human exposure to aflatoxin M1 and its association with risk factors of diabetes mellitus

    DOI: 10.1007/s11356-021-14871-w

    PMID: 34216342

    		 Sample: serum
  23. Journal of Diabetes Investigation (2021) IF: 4.232
    Diet-induced prediabetes: Effects on the activity of the renin-angiotensin-aldosterone system (RAAS) in selected organs

    DOI: 10.1111/jdi.13690
    Sample: liver
  24. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Zexie Tang targeting FKBP38/mTOR/SREBPs pathway improves hyperlipidemia

    DOI: 10.1016/j.jep.2022.115101

    PMID: 35151834

    		 Sample: serum
  25. TOXICOLOGY LETTERS (2021) IF: 4.374
    Alpha-lipoic acid attenuates silica-induced pulmonary fibrosis by improving mitochondrial function via AMPK/PGC1α pathway activation in C57BL/6J mice

    DOI: 10.1016/j.toxlet.2021.07.003

    PMID: 34252510

    		 Sample: serum
  26. JOURNAL OF PSYCHIATRIC RESEARCH (2022) IF: 4.791
    Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression

    DOI: 10.1016/j.jpsychires.2022.04.002

    PMID: 35429740

    		 Sample: serum
  27. Frontiers in Physiology (2019) IF: 3.201
    Dietary Bile Acids Enhance Growth, and Alleviate Hepatic Fibrosis Induced by a High Starch Diet via AKT/FOXO1 and cAMP/AMPK/SREBP1 Pathway in Micropterus salmoides

    DOI: 10.3389/fphys.2019.01430

    PMID: 31824338

    		 Sample: Plasma
  28. ANIMAL FEED SCIENCE AND TECHNOLOGY (2021) IF: 3.247
    Coating beef tallow with calcium soap improves dietary pellet quality and promotes an effective diet-induced obese mouse model

    DOI: 10.1016/j.anifeedsci.2021.115008
    Sample: plasma
  29. EUROPEAN JOURNAL OF PHARMACOLOGY (2020) IF: 3.263
    Pancreastatin inhibitor PSTi8 protects the obesity associated skeletal muscle insulin resistance in diet induced streptozotocin-treated diabetic mice

    DOI: 10.1016/j.ejphar.2020.173204
    Sample: plasma
  30. EUROPEAN JOURNAL OF PHARMACOLOGY (2020) IF: 3.263
    Paeonol prevents lipid metabolism dysfunction in palmitic acid-induced HepG2 injury through promoting SIRT1-FoxO1-ATG14-dependent autophagy

    DOI: 10.1016/j.ejphar.2020.173145
    Sample: HepG2 cell
  31. JOURNAL OF EXPERIMENTAL BIOLOGY (2021) IF: 3.312
    Monoterpenes alter TAR1-driven physiology in Drosophila species

    DOI: 10.1242/jeb.232116
  32. MEAT SCIENCE (2019) IF: 3.483
    Effects of naturally-produced lovastatin on carcass characteristics, muscle physico-chemical properties and lipid oxidation and cholesterol content in goats

    DOI: 10.1016/j.meatsci.2019.04.008
    Sample: serum
  33. Nutrients (2017) IF: 3.55
    Flavonoid-Rich Extract of Paulownia fortunei Flowers Attenuates Diet-Induced Hyperlipidemia, Hepatic Steatosis and Insulin Resistance in Obesity Mice by AMPK Pathway

    DOI: 10.3390/nu9090959
    Sample: Liver
  34. MOLECULAR AND CELLULAR ENDOCRINOLOGY (2018) IF: 3.563
    Curcumin restrains hepatic glucose production by blocking cAMP/PKA signaling and reducing acetyl CoA accumulation in high-fat diet (HFD)-fed mice

    DOI: 10.1016/j.mce.2018.02.018
    Sample: liver
  35. LIFE SCIENCES (2020) IF: 3.647
    Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis

    DOI: 10.1016/j.lfs.2020.118118
    Sample: serum
  36. JOURNAL OF PHARMACY AND PHARMACOLOGY (2021) IF: 3.765
    Cissus quadrangularis extract attenuates diabetic nephropathy by altering SIRT1/DNMT1 axis

    DOI: 10.1093/jpp/rgab078

    PMID: 34128987

    		 Sample: plasma
  37. Heliyon (2020) IF: 3.776
    PSTi8 with metformin ameliorates perimenopause induced steatohepatitis associated ER stress by regulating SIRT-1/SREBP-1c axis

    DOI: 10.1016/j.heliyon.2020.e05826
    Sample: plasma
  38. PEPTIDES (2022) IF: 3.867
    Pancreastatin inhibitor PSTi8 balances energy homeostasis by attenuating adipose tissue inflammation in high fat diet fed mice

    DOI: 10.1016/j.peptides.2022.170902
    Sample: serum
  39. Canadian Journal of Gastroenterology and Hepatology (2020) IF: 2.055
    Bredemolic Acid Ameliorates Selected Liver Function Biomarkers in a Diet-Induced Prediabetic Rat Model

    DOI: 10.1155/2020/2475301

    PMID: 32149046

    		 Sample: Tissue homogenate
  40. AQUACULTURE RESEARCH (2021) IF: 2.082
    Quillaja saponaria and/or Yucca schidigera ameliorate water quality, growth performance, blood health, intestine and gills histomorphology of Nile tilapia, Oreochromis niloticus

    DOI: 10.1111/are.15474
    Sample: serum
  41. THERIOGENOLOGY (2020) IF: 2.094
    Diet-induced obesity is associated with altered expression of sperm motility-related genes and testicular post-translational modifications in a mouse model

    DOI: 10.1016/j.theriogenology.2020.09.023

    PMID: 32980686

    		 Sample: Serum
  42. Journal of Developmental Origins of Health and Disease (2020) IF: 2.456
    Neonatal zingerone protects against the development of high-fructose diet-induced metabolic syndrome in adult Sprague-Dawley rats

    DOI: 10.1017/S2040174420000525
    Sample: plasma
  43. Cardiovascular Therapeutics (2020) IF: 2.538
    Bredemolic Acid Improves Cardiovascular Function and Attenuates Endothelial Dysfunction in Diet-Induced Prediabetes: Effects on Selected Markers

    DOI: 10.1155/2020/1936406

    PMID: 32117470

    		 Sample: Plasma,Tissue homogenate
  44. NUTRITION (2016) IF: 2.839
    Dietary sweet cherry anthocyanins attenuates diet-induced hepatic steatosis by improving hepatic lipid metabolism in mice

    DOI: 10.1016/j.nut.2016.01.007
    Sample: liver tissue
  45. International Journal of Clinical and Experimental Pathology (2018) IF: 1.396
    Pitavastatin attenuates atherosclerosis by suppressing NF-κB signaling in a high-cholesterol diet plus balloon catheter injury rabbit model

    PMID: 31938118

    		 Sample: serum
  46. AQUACULTURE RESEARCH (2020) IF: 1.748
    Dietary essential oil extract from sweet orange (Citrus sinensis) and bitter lemon (Citrus limon) peels improved Nile tilapia performance and health status

    DOI: 10.1111/are.15000
    Sample: serum
  47. Reports of Biochemistry and Molecular Biology (2021)
    The Impact of Intermittent Hypobaric Hypoxia Exposures on Triacylglycerol Synthesis in Rat Liver

    DOI: 10.52547/rbmb.10.3.437
    Sample: Liver tissue
  48. Heliyon (2021)
    Phyllanthus amarus attenuated derangement in renal-cardiac function, redox status, lipid profile and reduced TNF-α, interleukins-2, 6 and 8 in high salt diet fed rats

    DOI: 10.1016/j.heliyon.2021.e08106
    Sample: serum
  49. Veterinary World (2020)
    Cinnamomum burmannii (Nees & T. Nees) Blume and Eleutherine palmifolia (L.) Merr. extract combination ameliorate lipid profile and heart oxidative stress in hyperlipidemic mice

    DOI: 10.14202/vetworld.2020.1404-1409

    PMID: 32848317

    		 Sample: Serum
  50. Journal of Advanced Pharmaceutical Technology & Research (2020)
    Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats

    DOI: 10.4103/japtr.JAPTR_28_20
    Sample: serum
  51. Revista Brasileira de Medicina do Esporte (2022) IF: 0.652
    IMPACTS OF AEROBIC EXERCISE ON THE OBESITY OF ADOLESCENTS AND THEIR LIPID METABOLISM

    DOI: 10.1590/1517-8692202329012022_0163
    Sample: serum
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

E******************eSubmitted [ Nov 02 2023 ]

Asked: Does your triglyceride quantification kit (E-BC-K238) include what is needed to prepare the standard curve before measuring absorbance of the actual samples?

Reply

A

adminSubmitted [ Nov 02 2023 ]

Answered: This kit does not need to make standard curve, but only needs to make a concentration of the standard well. Besides, the kit contains the standard reagent.

Q

M*****aSubmitted [ Sep 23 2022 ]

Asked: What cell culture media and composition would you recommend for TG assays. I would like to look at the cell culture supernatant. Thank you

Reply

A

adminSubmitted [ Sep 23 2022 ]

Answered: Sorry, our TG Assay Kit can not be used on cell culture supernatant.

Q

M*****aSubmitted [ Aug 31 2022 ]

Asked: Triglyceride Colorimetric Assay (E-BC-K238) Just wanted to find out if measure the OD value at 492nm instead of 510nm? We don’t have the 510nm filter

Reply

A

adminSubmitted [ Aug 31 2022 ]

Answered: Yes, it can be replaced with 492nm. It doesn't matter.

Q

Y***********oSubmitted [ Jun 25 2018 ]

Asked: I saw \"for in vitro determination of TG content\". I\'m looking for a in vivo TG content kit for mouse skeletal muscle tissue. In manual, you suggested that \"tissue sample\" uses in sample preparation. Therefore, please request my question.

Reply

A

adminSubmitted [ Jun 25 2018 ]

Answered: For item E-BC-K238 Triglyceride Assay Kit (Single reagent, GPO-PAP Method), it applies to the animal tissue for in vitro determation, rather than in vivo animal. Hope it is helpful for you.

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