TAL can decompose tyrosine to produce 4-coumaric acid, which has a strong absorption peak at 333 nm. Therefore, the activity of TAL can be calculated by measuring the OD value at 333 nm.

Performance characteristics

Synonyms	TAL
Sample type	fruit juices, plant and animal tissue
Sensitivity	0.12 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	70 min
Other instruments required	Micropipette, Incubator, Centrifuge, Water bath
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment.

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
20% Epipremnum aureum tissue homogenate	1
20% Celery tissue homogenate	1
20% Cilantro tissue homogenate	1
20% Rat liver tissue homogenate	1
20% Rat kidney tissue homogenate	1
20% Corn tissue homogenate	1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

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Cat. No	E-BC-K851-M
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