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Vitamin C (VC) Colorimetric Assay Kit

  • Cat.No.:E-BC-K034-M

  • Detection instrument: Microplate reader(530-540 nm,optimum wavelength: 536 nm)

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Price: $200

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Detection principle

The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.

Performance characteristics

Synonyms VC,Ascorbic acid
Sample type Serum,plasma,tissue
Sensitivity 0.31 μg/mL
Detection range 0.31-20 μg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 50min
Precision Average inter-assay CV: 6.1%Average intra-assay CV: 2.2%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Absolute ethanol
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.31-20 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Mouse serum


Chicken serum


Horse serum


10% Mouse kidney tissue homogenate


10% Mouse liver tissue homogenate


10% Mouse lung tissue homogenate


10% Mouse spleen tissue homogenate


10% Rat liver tissue homogenate


10% Rat heart tissue homogenate


10% Plant tissue homogenate


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Ralated product

Cat Number Product Name
E-BC-K033-M Vitamin E (VE) Colorimetric Assay Kit


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