For research use only.
| Detection Principle | Fe<sup>3+</sup> can be deoxidized to Fe<sup>2+</sup> by VE with ferroin existing. Fe<sup>2+</sup> can react with phenanthroline and form pink compound under certain condition. After colorimetric assay, VE content can be figured out according to the standard curve or calculated through formula. |
| Synonyms | VE |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (525-533 nm, optimum wavelength: 533 nm) |
| Research Area | Oxidative Stress , Plant Physiology Series |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4), Absolute ethanol, N-heptane |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.95 μg/mL |
| Detection Range | 0.95-40 μg/mL |
| Precision | inter-assay CV: 6.3% | intra-assay CV: 3.9% |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| Chicken serum | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat heart tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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