Vitamin C (VC) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K034-S

      • 100Assays
      • 50Assays
      - +
      Price: $200
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(533 nm)

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      Detection principle

      The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.

      Performance characteristics

      Synonyms VC,Ascorbic acid
      Sample type Serum,plasma,tissue
      Sensitivity 0.35 μg/mL
      Detection range 0.35-20 μg/mL
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 80 min
      Precision Average inter-assay CV: 3.3%Average intra-assay CV: 2.3%
      Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
      Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol
      Storage 2-8℃
      Valid period 6 months


      1. Cold plasma seed priming modulates growth, redox homeostasis and stress response by inducing reactive species in tomato (Solanum lycopersicum)
        IF: 6.17
        Journal: Free Radical Biology and Medicine(2020)
        DOI: 10.1016/j.freeradbiomed.2020.06.003
        PMID: 32561321
        Products: E-BC-K034-S 
        Sample type: Plant?Tissue
        Reactivity: Other Plants
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      MSDS for ELISA ISO 9001

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