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Vitamin C (VC) Colorimetric Assay Kit

  • Cat.No.:E-BC-K034-S

  • Detection instrument: Spectrophotometer(533 nm)

To Purchase E-BC-K034-S

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  • 100Assays
  • 50Assays
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Detection principle

The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.

Performance characteristics

Synonyms VC,Ascorbic acid
Sample type Serum,plasma,tissue
Sensitivity 0.35 μg/mL
Detection range 0.35-20 μg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 3.3%Average intra-assay CV: 2.3%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol
Storage 2-8℃
Valid period 6 months

Images

The ascorbate concentration in tomato is significantly reduced after being treated with cold pla sma for 1, 5 and 10 minutes respectively. (P<0.0001)

B Adhikari et al investigate the effect of cold plasma in priming modulates growth, redox homeostasis and stress in tomato. Vitamin C level of tomato was determined using VC colorimetric assay kit (E-BC-K034-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.35-20 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

10% Mouse kidney tissue homogenization

1

Rat serum

1

Mouse serum

1

10% Rat liver tissue homogenization

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Free Radical Biology and Medicine (2020) IF: 6.17
    Cold plasma seed priming modulates growth, redox homeostasis and stress response by inducing reactive species in tomato (Solanum lycopersicum)

    DOI: 10.1016/j.freeradbiomed.2020.06.003

    PMID: 32561321

    Sample: Plant?Tissue

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