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Vitamin C (VC) Colorimetric Assay Kit

  • Cat.No.:E-BC-K034-S

  • Detection instrument: Spectrophotometer(533 nm)

To Purchase E-BC-K034-S

  • 100Assays
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Price: $200

Detection principle

The most obvious chemical activity of VC is that reduce Fe3+ to Fe2+, then promote iron absorption in the intestine, promote the storage and utilization of iron. Fe3+ react immediately with reducing ascorbic acid to form Fe2+. then Fe2+ react with phenanthroline and the color developing reaction occurs. The content of vitamin C in sample can be determined. Measure the OD value and calculate the VC content indirectly.

Performance characteristics

Synonyms VC,Ascorbic acid
Sample type Serum,plasma,tissue
Sensitivity 0.35 μg/mL
Detection range 0.35-20 μg/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 3.3%Average intra-assay CV: 2.3%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Absolute ethanol
Storage 2-8℃
Valid period 6 months


The ascorbate concentration in tomato is significantly reduced after being treated with cold pla sma for 1, 5 and 10 minutes respectively. (P<0.0001)

B Adhikari et al investigate the effect of cold plasma in priming modulates growth, redox homeostasis and stress in tomato. Vitamin C level of tomato was determined using VC colorimetric assay kit (E-BC-K034-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.35-20 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Human plasma


10% Mouse kidney tissue homogenization


Rat serum


Mouse serum


10% Rat liver tissue homogenization


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Free Radical Biology and Medicine (2020) IF: 6.17
    Cold plasma seed priming modulates growth, redox homeostasis and stress response by inducing reactive species in tomato (Solanum lycopersicum)

    DOI: 10.1016/j.freeradbiomed.2020.06.003

    PMID: 32561321

    Sample: Plant?Tissue


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