For research use only.
| Verified Samples |
Verified Samples in WB: 3T3, HT29 Verified Samples in IHC: Mouse brain |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000 |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Wnt-1 |
| Abbre | Wnt-1 |
| Synonyms | BMND16, INT1, OI15, Proto oncogene protein Wnt 1, Proto-oncogene Int-1 homolog, Proto-oncogene Wnt-1, WNT1, Wingless type MMTV integration site family member 1, Wnt 1, member 1 (oncogene INT1), oncogene Int1, wingless-type MMTV integration site family, wnt1 |
| Swissprot | |
| Calculated MW | 41 kDa |
| Observed MW |
45 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted>extracellular space>extracellular matrix. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. |
Other Clones
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