AK6 Polyclonal Antibody (E-AB-40266)
For research use only.
| Verified Samples |
Verified Samples in WB: Rat testis |
| Dilution | WB 1:1000-1:4000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Rat Adenylate kinase isoenzyme 6 protein |
| Abbre | AK6 |
| Synonyms | AD-004, AK/ATPase, AK6, Adenylate kinase isoenzyme 6, Adrenal gland protein AD-004, CGI-137, CIP, Coilin-interacting nuclear ATPase protein, Dual activity adenylate kinase/ATPase, KAD6, TAF9, hCINAP |
| Swissprot | |
| Calculated MW | 20 kDa |
| Observed MW |
19 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus>nucleoplasm. Nucleus>Cajal body. Displays widespread diffuse nucleoplasmic distribution but not detected in nucleoli. Detected in Cajal bodies but not in all cells. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a protein that belongs to the adenylate kinase family of enzymes. The protein has a nuclear localization and contains Walker A (P-loop) and Walker B motifs and a metal-coordinating residue. The protein may be involved in regulation of Cajal body formation. In human, AK6 and TAF9 (GeneID: 6880) are two distinct genes that share 5' exons. Alternative splicing results in multiple transcript variants.AK6 (Adenylate Kinase 6) is a Protein Coding gene. Among its related pathways are Ribosome biogenesis in eukaryotes and Purine metabolism (REACTOME). GO annotations related to this gene include ATPase activity and adenylate kinase activity. |
Other Clones
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Unconjugated
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