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For research use only.

Verified Samples Verified Samples in WB: various cell lines
Verified Samples in IHC: Human placenta, Rat ovary
Verified Samples in IF: HeLa
Dilution WB 1:1000-1:2000,  IHC 1:50-1:200,  IF 1:50-1:200
Isotype IgG
Host Rabbit
Reactivity Human,  Rat
Applications WB,  IHC,  IF
Clonality Polyclonal
Immunogen Recombinant fusion protein of human Aromatase
Abbre Aromatase
Synonyms ARO,  ARO1,  Aromatase,  CPV1,  CYAR,  CYP19,  CYP19A1,  CYPXIX,  P-450AROM,  aromatase
Swissprot
Calculated MW 24 kDa/57 kDa
Observed MW 48 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Membrane, Peripheral membrane protein.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Cell Biology,  Epigenetics and Nuclear Signaling,  Metabolism,  Neuroscience
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities.
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Unconjugated

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