Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit (Aspartate Substrate Method) (E-BC-K1301-M)

Name: Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit (Aspartate Substrate Method)
Brand: Elabscience
SKU: E-BC-K1301-M
Price: 150.0 USD
Availability: InStock

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Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit (Aspartate Substrate Method) - 1

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	96T	$ 220.00	- +
	48T	$ 150.00	- +

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Detection Principle	Glutamic-oxaloacetic transaminase (GOT), also known as aspartate aminotransferase (AST), is an important indicator reflecting liver function. AST in the sample catalyzes the deamination of L-aspartic acid to produce oxaloacetic acid. Oxaloacetic acid is reduced by the enzyme while NADH is oxidized to NAD+, resulting in a decrease in the absorbance at 340 nm. By monitoring the rate of the decrease in absorbance at 340 nm, the enzymatic activity of AST can be determined.
Sample Type	Animal tissue,Cell,Serum,Plasma
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (340 nm)
Research Area	Liver And Renal Function
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	5.81 U/L
Detection Range	5.81-1161.13 U/L
Precision	inter-assay CV: 2.1%-5.8% \| intra-assay CV: 2.6%-4.8%
Sample Volume	10 μL
Assay Time	30 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Human plasma	1
Mouse serum	1
10% Mouse liver tissue homogenate	20-50
10% Mouse kidney tissue homogenate	20-50
10% Mouse heart tissue homogenate	20-50
1×10^6 K562 cells	1
1×10^6 HL-60 cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.