Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit (Aspartate Substrate Method) (E-BC-K1301-M)
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For research use only.
| Detection Principle | Glutamic-oxaloacetic transaminase (GOT), also known as aspartate aminotransferase (AST), is an important indicator reflecting liver function. AST in the sample catalyzes the deamination of L-aspartic acid to produce oxaloacetic acid. Oxaloacetic acid is reduced by the enzyme while NADH is oxidized to NAD+, resulting in a decrease in the absorbance at 340 nm. By monitoring the rate of the decrease in absorbance at 340 nm, the enzymatic activity of AST can be determined. |
| Sample Type | Animal tissue,Cell,Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 5.81 U/L |
| Detection Range | 5.81-1161.13 U/L |
| Precision | inter-assay CV: 2.1%-5.8% | intra-assay CV: 2.6%-4.8% |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| 10% Mouse liver tissue homogenate | 20-50 |
| 10% Mouse kidney tissue homogenate | 20-50 |
| 10% Mouse heart tissue homogenate | 20-50 |
| 1×10^6 K562 cells | 1 |
| 1×10^6 HL-60 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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