beta Tubulin Monoclonal Antibody (AN005310L)
For research use only.
| Verified Samples |
Verified Samples in WB: HT-29, Jurkat, 293T, Raw264.7, C6, Rat brain, Zebrafish Verified Samples in IF: U-2 OS |
| Dilution | WB 1:2000-1:4000, IF 1:200-1:400 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat, Zebrafish |
| Applications | WB, IF |
| Clonality | Monoclonal |
| Immunogen | Recombinant human TUBB protein by E.coli |
| Abbre | beta Tubulin |
| Synonyms | Beta 4 tubulin, Beta 5 tubulin, beta Ib tubulin, Beta1 tubulin, Class I beta tubulin, TUBB 1, TUBB 2, TUBB 5, TUBB, TUBB1, TUBB2, TUBB5, tubulin beta 1 chain, Tubulin beta 2 chain, tubulin beta 5 chain, Tubulin beta chain, Tubulin beta class I, tubulin beta polypeptide, Tubulin beta-5 chain |
| Swissprot | |
| Calculated MW | 55 kDa |
| Observed MW |
55 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, cytoskeleton |
| Tissue Specificity | Ubiquitously expressed with highest levels in spleen, thymus and immature brain. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Signal Transduction, Neuroscience, Tags and Cell Markers, Isotype/Loading Controls, Biochemicals, Cancer, Cardiovascular, Cell Biology, Developmental Biology, Epigenetics and Nuclear Signaling, Immunology, Metabolism, Microbiology, Stem Cells |
| Clone No. | 2E8-2 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | In eukaryotes there are six members of the tubulin superfamily. α- and β-tubulins polymerize into microtubules,a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes,including mitosis. Both α and β tubulins have a mass of around 50 kDa. Tubulin was long thought to be specific to eukaryotes. Beta-tubulin gene is commonly used as a loading control for western blot. |
Other Clones
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Other Formats
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Elab Fluor®488
HRP
Unconjugated
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