Betacellulin/BTC Polyclonal Antibody (AN005970L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse pancreas, Rat pancreas |
| Dilution | WB 1:1000-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Mouse Betacellulin/BTC protein expressed by E.coli |
| Abbre | Betacellulin/BTC |
| Synonyms | Bcn, Btc |
| Swissprot | |
| Calculated MW | 19 kDa |
| Observed MW |
32 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted, Cell membrane |
| Tissue Specificity | Found in several mouse tissues including kidney, uterus and liver, as well as in beta tumor cell line and MCF-7 cells. It is not detected in the brain. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, was originally isolated from mouse beta cell tumor (betaTC-3) as a growth–promoting factor and is known to be expressed in pancreatic α-cells, β-cells, and duct cells in adult humans. BTC acts through binding to four ErbB tyrosine kinase receptors (EGF-R/ErbB-1, neu/ErbB-2, ErbB-3, and ErbB-4). |
Other Clones
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Other Formats
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Unconjugated
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