Bradford Protein Colorimetric Assay Kit (E-BC-K168-S)
For research use only.
| Detection Principle | Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm. |
| Synonyms | TP |
| Sample Type | Serum,Plasma,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (595 nm) |
| Research Area | Amino Acids And Proteins , Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/room temperature for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.026 mg/mL |
| Detection Range | 0.026-1.2 mg/mL |
| Precision | inter-assay CV: 2.3% | intra-assay CV: 1.8% |
| Sample Volume | 50 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 40-60 |
| 10% Mouse liver tissue homogenization | 8-12 |
| Rat serum | 40-60 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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