Biuret Protein Colorimetric Assay Kit (E-BC-K165-S)
For research use only.
| Detection Principle | Any compound that contains two -CONH2 in the molecule can react with alkaline copper solution to form a purple complex, which is known as the biuret reaction. Many peptide bonds (-CONH-) in protein molecules can perform this reaction, and the color degree of all kinds of proteins are essentially the same. |
| Synonyms | TP |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (540 nm) |
| Research Area | Amino Acids And Proteins , Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.373 g/L |
| Detection Range | 0.373-80 g/L |
| Precision | inter-assay CV: 2.6% | intra-assay CV: 1.2% |
| Sample Volume | 50 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 2-4 |
| Porcine serum | 2-4 |
| 10% Epipremnum aureum tissue homogenate | 1 |
| 5% Mouse liver tissue homogenate | 1 |
| 5% Mouse heart tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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