Caspase-9 Monoclonal Antibody (AN007230L)
For research use only.
| Verified Samples | Verified Samples in WB: Hep G2, HeLa, THP-1 |
| Dilution | WB 1:250-1:500 |
| Isotype | IgG2a |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human Caspase-9 protein expressed by E.coli |
| Abbre | Caspase-9 |
| Synonyms | Apoptosis related cysteine peptidase, Apoptotic protease-activating factor 3, Caspase 9 apoptosis related cysteine peptidase, Caspase 9 Dominant Negative, Caspase 9c, Caspase-9 subunit p10, ICE LAP6, ICE like apoptotic protease 6, ICE-like apoptotic protease 6, protein phosphatase 1, regulatory subunit 56, RNCASP9, MCH, Apoptotic protease Mch, PPP1R, ICE-LAP, CASP9, APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56, caspase-9, Caspase 9, CASP-9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3 (APAF-3), ICE-like apoptotic protease 6 (ICE-LAP6) |
| Swissprot | |
| Calculated MW | 46 kDa |
| Observed MW |
35 kDa;47 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus, Mitochondrion |
| Tissue Specificity | Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Protein A/G Purification |
| Research Areas | Cell Biology, Cancer, Metabolism |
| Clone No. | 3B6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
Other Clones
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Unconjugated
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