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For research use only.

Verified Samples Verified Samples in WB: Human brain
Verified Samples in IHC: Human lung cancer, Human liver cancer
Dilution WB 1:500-1:2000,  IHC 1:30-1:150
Isotype IgG
Host Rabbit
Reactivity Human
Applications WB,  IHC
Clonality Polyclonal
Immunogen Synthetic peptide of human CDT1
Abbre CDT1
Synonyms CDT 1,  CDT1,  Chromatin licensing and DNA replication factor 1,  DNA replication factor,  DNA replication factor Cdt1,  DUP,  Double parked,  Double parked Drosophila homolog of,  Double parked homolog,  Ret,  Retroviral integration site 1,  Retroviral integration site 2,  cdt1
Swissprot
Calculated MW 60 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus.
Concentration 0.7 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method Antigen affinity purification
Research Areas Cancer,  Epigenetics and Nuclear Signaling
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein.
Other Clones

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Unconjugated

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