Cellular Cuprous Fluorometric Assay Kit (E-BC-F102)

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

All	Size	Price	Qty
	96T	$ 480.00
	48T	$ 350.00

Bulk request >>

Add to cart

For research use only. Order now, ship in 3 days

Detection Principle	Abnormal accumulation of cellular cuprous in cells induces the oligomerization of key components of the lipoic acid-modified pyruvate dehydrogenase complex, which affects the tricarboxylic acid cycle, triggers proteotoxic stress, and induces cell death. Cellular cuprous can catalyze the substrate to produce fluorescent substances, and the higher the copper ion concentration, the more fluorescent substances are generated per unit time. The fluorescence delete can be detected at the excitation wavelength of 395 nm and emission wavelength of 480 nm.
Sample Type	Cell
Detection Method	Fluorometric method
Detection Instrument	Fluorescence microplate reader (Ex/Em=395 nm/480 nm)
Research Area	Cuproptosis
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Precision	inter-assay CV: 5.0-8.0% \| intra-assay CV: 3.0-6.0%
Assay Time	50 min

Cat.No.	Product Name	Sizes
E-BC-F047	α-Ketoglutarate (α-KG) Fluorometric Assay Kit	96T , 48T
E-BC-K083-M	α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit	96T
E-BC-K138-F	Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green)	96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
1×10^6 Hela cells	1
1×10^6 Jurkat cells	1
1×10^6 K562 cells	1
1×10^6 Molt-4 cells	1
1×10^6 293T cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.