Cellular Cuprous Fluorometric Assay Kit (E-BC-F102)
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For research use only.
| Detection Principle |
Abnormal accumulation of cellular cuprous in cells induces the oligomerization of key components of the lipoic acid-modified pyruvate dehydrogenase complex, which affects the tricarboxylic acid cycle, triggers proteotoxic stress, and induces cell death. Cellular cuprous can catalyze the substrate to produce fluorescent substances, and the higher the copper ion concentration, the more fluorescent substances are generated per unit time. The fluorescence delete can be detected at the excitation wavelength of 395 nm and emission wavelength of 480 nm. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=395 nm/480 nm) |
| Research Area | Cuproptosis |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 5.0-8.0% | intra-assay CV: 3.0-6.0% |
| Sample Volume | 20 μL(cell homogenate) |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 1×10^6 Hela cells | 1 |
| 1×10^6 Jurkat cells | 1 |
| 1×10^6 K562 cells | 1 |
| 1×10^6 Molt-4 cells | 1 |
| 1×10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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