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For research use only.

Verified Samples Verified Samples in WB: PC-3
Verified Samples in IHC: Human duodenum, Mouse small intestine, Mouse pancreas
Dilution WB 1:500-1:1000,  IHC 1:100-1:300
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse
Applications WB,  IHC
Clonality Polyclonal
Immunogen Recombinant Mouse Chromogranin-A protein
Abbre Chromogranin A
Synonyms CHG A,  CMGA,  CgA,  Chga,  Chromogranin A,  Chromogranin A parathyroid secretory protein 1,  Chromogranin A precursor,  ChromograninA,  ER-37,  Pancreastatin,  Parastatin,  Parathyroid secr,  beta Granin,  betagranin (N-terminal fragment of chromogranin A),  catestatin,  chromofungin
Swissprot
Calculated MW 51 kDa
Observed MW 51 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasmic vesicle, Membrane, Secreted.
Concentration 1 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Antigen Affinity Purification
Research Areas Cancer,  Neuroscience,  Signal Transduction,  Tags and Cell Markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin,pancreastatin,catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) ,and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.
Other Clones

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Unconjugated

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