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For research use only.
| Verified Samples |
Verified Samples in WB: Human Hela, Human A431 Verified Samples in IHC: Human liver cancer, Human lung cancer, Human colon cancer |
| Dilution | WB 1:5000-1:10000, IHC 1:500-1:1000 |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human KRT18 protein expressed by E.coli |
| Abbre | CK |
| Synonyms | CK 18, CK-18, CK18, CYK 18, CYK18, Cell proliferation inducing gene 46 protein, Cell proliferation inducing protein 46, Cell proliferation-inducing gene 46 protein, Cytokeratin 18, Cytokeratin endo B, Cytokeratin-18, K 18, K18, K1C18, KA18, Keratin 18, type I |
| Swissprot | |
| Calculated MW | 48 kDa |
| Observed MW |
45 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Signal Transduction, Tags and Cell Markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Cytokeratin 18 (CK18) has a molecular weight of 45 KDa, which is a low molecular weight type A cytokeratin. It is a marker of simple epithelium, including glandular epithelium. The stratified squamous epithelium is generally negative. CK18 is mainly used in the diagnosis of adenocarcinoma. |
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