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For research use only.

Verified Samples Verified Samples in WB: Hela, HepG2, SW480
Verified Samples in IHC: Mouse heart, Rat heart
Dilution WB 1:1000-1:2000,  IHC 1:300-1:800
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC
Clonality Polyclonal
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse Connexin 43
Abbre Connexin 43
Synonyms CXA1,  Connexin 43,  Connexin-43,  Cx 43,  Cx43,  DFNB38,  GJA 1,  GJAL,  Gap junction 43 kDa heart protein,  Gap junction alpha-1 protein,  Gap junction protein alpha 1 43kDa,  Gap junction protein alpha 1 43kDa (connexin 43),  Gap junction protein alpha like,  Gja1,  ODD,  ODDD,  ODOD
Swissprot
Calculated MW 43 kDa
Observed MW 43 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cell membrane. Cell junction>gap junction.
Concentration 0.53 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Cardiovascular,  Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations.
Other Clones

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Unconjugated

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