Toll-free:1-888-852-8623

All categories

  • All categories
  • Flow Cytometry Antibodies
  • ELISA Kits
  • MACS Cell Isolation
  • Antibodies and Reagents
  • Apoptosis and Cell Health Detection
  • Metabolism Assays
  • Immunoassays
  • Cell Identification Kits
  • Proteins and Peptides
  • Cell Culture
Please enter the item number/product keyword!
Keyword cannot be empty !
INSERT SYMBOLS:
  • α
  • β
  • γ
  • δ
  • ε
  • ζ
  • η
  • θ
  • κ
  • μ
  • ω
  • σ
  • τ
  • λ
  • ⅩⅢ
  • ⅩⅢ
  • ⅩⅣ
  • ⅩⅤ
  • ⅩⅦ
  • ⅩⅧ
  • UP ↑
  • +1
All Size Price Qty
200μL $ 530.00
120μL $ 320.00
60μL $ 200.00
Add to cart

For research use only.

Verified Samples Verified Samples in WB: Mouse brain, Rat brain
Verified Samples in IHC: Rat liver, Mouse liver, Mouse spinal cord
Dilution WB 1:200-1:2000,  IHC 1:50-1:200
Isotype IgG
Host Rabbit
Reactivity Mouse,  Rat
Applications WB,  IHC
Clonality Polyclonal
Immunogen Recombinant fusion protein of human CTNNA2 (NP_004380.2).
Abbre CTNNA2
Synonyms CAP-R,  CAPR,  CT114,  CTNNA2,  CTNR
Swissprot
Calculated MW 59 kDa/65 kDa/95 kDa/100 kDa/104 kDa/105 kDa
Observed MW 120 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm, Cytoplasm, cytoskeleton, Cell junction, adherens junction, Cell membrane, Cell projection, axon, Recruited to the nucleus upon ZNF639 overexpression.
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method Affinity purification
Research Areas Cancer,  Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background CTNNA2 (Catenin Alpha 2) is a Protein Coding gene. Diseases associated with CTNNA2 include Cortical Dysplasia, Complex, With Other Brain Malformations 9 and Arrhythmogenic Right Ventricular Cardiomyopathy. Among its related pathways are Blood-Brain Barrier and Immune Cell Transmigration: VCAM-1/CD106 Signaling and Gastric cancer. Gene Ontology (GO) annotations related to this gene include structural molecule activity and structural constituent of cytoskeleton. An important paralog of this gene is CTNNA1.
Other Clones

{{antibodyDetailsPage.numTotal}} Results

Other Formats

{{formatDetailsPage.numTotal}} Results

Unconjugated

  • IF:{{item.impact}}

    Journal:{{item.journal}} ({{item.year}})

    DOI:{{item.doi}}

    Reactivity:{{item.species}}

    Sample Type:{{item.organization}}

  • Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}