For research use only.
| Verified Samples | Verified Samples in WB: Human HepG2 |
| Dilution | WB 1:500-1:1000 |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human CTSB protein expressed by Mammalian |
| Abbre | CTSB |
| Synonyms | APP secretase, APPS, Amyloid precursor protein secretase, CATB, CPSB, CTSB, Cathepsin B heavy chain, Cathepsin B1, CathepsinB, OTTHU, OTTHUMP00000116009, OTTHUMP00000229510, OTTHUMP00000229511, OTTHUMP00000229512, OTTHUMP00000229514, OTTHUMP00000229515, cysteine protease |
| Swissprot | |
| Calculated MW | 38 kDa |
| Observed MW |
44/27/24 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Lysosome Melanosome Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer, Neuroscience, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains,both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease,the most common cause of dementia. Overexpression of the encoded protein,which is a member of the peptidase C1 family,has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. |
Other Clones
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