Cystatin C Polyclonal Antibody(Capture/Detector) (AN000020P)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, PC-3, CaCo2, Mouse kidney, Rat kidney, Rat liver, Rat plasma Verified Samples in ELISA: Recombinant Human Cystatin C protein, Human serum, Human plasma |
| Dilution | WB 1:1000-1:2000, ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA Capture/Detector |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human Cystatin C protein expressed by E.coli |
| Abbre | Cystatin C |
| Synonyms | Cystatin-C, CST3, CYTC, Cystatin-3, Gamma-trace, Neuroendocrine basic poLypeptide, Post-gamma-gLobuLin |
| Swissprot | |
| Calculated MW | 13 kDa |
| Observed MW |
13 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Tags & Cell Markers, Cell Biology, Stem Cells, Cardiovascular |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Cystatin C is a member of family 2 of the Cystatin superfamily . It is involved in processes such as tumor invasion and metastasis,inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B,H,K,l,and S. |
Other Clones
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Unconjugated
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