Dual Luciferase Reporter Gene Luminescence Assay Kit (Flash Type) (E-BC-F071)
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For research use only.
| Detection Principle |
Dual luciferase reporter gene uses luciferin to detect the luminescence reaction produced by luciferin substrate. The detection system includes two parts: firefly luciferase reporter gene and renilla luciferase reporter gene. As an internal parameter of transfection, the renilla luciferase can normalize the experimental data. The reaction principle of this test box is that in the presence of oxygen, ATP and magnesium ions at the same time, firefly luciferase and coelentin are catalyzed and oxidized successively by firefly luciferase and renilla luciferase in the samples to produce different fluorescence signals, and the expression levels of firefly luciferase and renilla luciferase in the samples can be detected by chemiluminescence instrument. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Chemiluminescence immunoassay analyzer or multifunctional microplate reader (with the function of detecting luminescence) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Assay Time | 50 min |
| Cat.No. | Product Name | Clone No. |
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