EIF4A1 Monoclonal Antibody (E-AB-22076)
For research use only.
| Verified Samples |
Verified Samples in WB: 293T, Hela, HepG2, Mouse brain Verified Samples in IHC: Mouse colon Verified Samples in IF: Mouse spleen |
| Dilution | WB 1:500-1:3000, IHC 1:50-1:300, IF 1:100-1:200 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-p, IF |
| Clonality | Monoclonal |
| Immunogen | Synthetic Peptide |
| Abbre | eIF4A1 |
| Synonyms | ATP-dependent RNA helicase eIF4A-1, DDX2, DDX2A, EIF4A, EIF4A1, Eukaryotic initiation factor 4A-I, Eukaryotic initiation factor 4AI, Eukaryotic translation initiation factor , eIF 4A I, eIF-4A-I, eIF4A I, eIF4A-I, eukaryotic translation initiation factor 4A, isoform 1 |
| Swissprot | |
| Observed MW |
48 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytosol, Extracellular region or secreted, extracellular exosome, extracellular matrix, Other locations: cytoplasm, eukaryotic translation initiation factor 4F complex, membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Protein A purification |
| Research Areas | Cancer, Epigenetics and Nuclear Signaling |
| Clone No. | 2B6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | EIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. |
Other Clones
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Other Formats
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Unconjugated
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