EMAPII/AIMP1 Monoclonal Antibody (AN200182P)
For research use only.
| Verified Samples | Verified Samples in WB:?HepG2 |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human EMAPII/AIMP1 Protein |
| Abbre | AIMP1 |
| Synonyms | HLD, AIMP, EMAP, SCYE, AIMP1, EMAP2, EMAPII, HLD3, SCYE1, p43, AIMP 1, AIMP1/p43, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1, ARS interacting multifunctional protein 1, EMAP 2, EMAP-2, EMAP-II, Endothelial monocyte activating polypeptide, Endothelial monocyte activating polypeptide 2, Endothelial monocyte-activating polypeptide 2, Endothelial monocyte-activating polypeptide II, formerly, member 1, member 1 (endothelial monocyte-activating), Multisynthase complex auxiliary component p43, Multisynthetase complex auxiliary component p43, OTTHUMP00000219673, Scye 1, Small inducible cytokine subfamily E, Small inducible cytokine subfamily E member 1 |
| Swissprot | |
| Calculated MW | 34 kDa |
| Observed MW |
36 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Cardiovascular, Immunology, Tags & Cell Markers, Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | 6A3 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
