Firefly Luciferase Reporter Gene Luminescence Assay Kit (Glow Type) (E-BC-F075)
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For research use only.
| Detection Principle |
Firefly Luciferin reporter gene detection is a reporting system that uses luciferin as a substrate to detect the activity of Firefly luciferase. The detection principle of this kit: In the presence of oxygen, ATP and magnesium ions at the same time, firefly luciferin is catalyzed by firefly luciferase in the sample to oxidize luciferin and emit yellow-green light, and the expression of firefly luciferin in the sample can be detected by the chemiluminescence instrument. Compared with E-BC-F072, the luminescence performance of this kit is longer and more stable, and it is suitable for the detection of this system with higher expression levels and diversity. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Chemiluminescence immunoassay analyzer or multifunctional microplate reader (with the function of detecting luminescence) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 4.7-6.9% | intra-assay CV: 1.2-3.4% |
| Assay Time | 30 min |
| Cat.No. | Product Name | Clone No. |
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