GLUT-3 Polyclonal Antibody (E-AB-31557)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse brain |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-p |
| Clonality | Polyclonal |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Glut3 |
| Abbre | GLUT-3 |
| Synonyms | FLJ90380, GLUT 3, GLUT-3, GLUT3, GTR3, Glucose transporter type 3, Glucose transporter type 3 brain, Slc2a3, Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3, Solute carrier family 2, brain, facilitated glucose transporter member 3 |
| Swissprot | |
| Calculated MW | 54 kDa |
| Observed MW |
55 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Metabolism, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Glucose transporter 3(or GLUT3), also known assolute carrier family 2, facilitated glucose transporter member 3(SLC2A3) is aproteinthat in humans is encoded by theSLC2A3gene. GLUT3facilitatesthe transport ofglucoseacross theplasma membranesof mammalian cells. GLUT3 is most known for its specific expression inneuronsand has originally been designated as the neuronal GLUT. GLUT3 has been studied in other cell types with specific glucose requirements, includingsperm, preimplantationembryos, circulatingwhite blood cellsandcarcinomacell lines. GLUT3 has both a higher affinity for glucose and at least a fivefold greater transport capacity than GLUT1, GLUT2 and GLUT4, which is particularly significant for its role in neuronal glucose transport, where ambient glucose levels are fivefold lower than in serum. |
Other Clones
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Other Formats
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Unconjugated
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