Glutathione Peroxidase (GSH-Px) Activity Assay Kit (E-BC-K096-M)
For research use only.
| Detection Principle | Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH. |
| Detection Principle Image | |
| Synonyms | GSH-PX |
| Sample Type | Serum,Plasma,Cell,Cell culture supernatant,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-420 nm, optimum wavelength: 412 nm) |
| Research Area | Oxidative Stress , Plant Physiology Series , Ferroptosis |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 34.34 U |
| Detection Range | 34.34-1036.64 U |
| Precision | inter-assay CV: 8.7% | intra-assay CV: 2.4% |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 3-5 |
| Rat serum | 5-8 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 30-60 |
| HepG2 cells (5 mgprot/mL) | 1 |
| 10% Epipremnum aureum leaves tissue homogenate | 1 |
| 10% Chinese cabbage leaves tissue homogenate | 3-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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