Granule-Bound Starch Synthase (GBSS) Activity Colorimetric Assay Kit (E-BC-K1203-M)
For research use only.
| Detection Principle | Granule-bound Starch Synthase (GBSS) catalyzes the reaction between ADPG and the primer, transferring glucose molecules to the primer and generating ADP, which in turn catalyzes the reduction of NADP+ to NADPH. The amount of NADPH produced was proportional to the amount of ADP produced in the previous reaction step, and GBSS activity was calculated by measuring the increase in NADPH at 340 nm. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 83 U/g wet weight |
| Detection Range | 83-6640 U/g wet weight |
| Precision | inter-assay CV: 2.8-6.3% | intra-assay CV: 1.4-3.1% |
| Sample Volume | 100 μL(tissue homogenate) |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Potato tissue homogenate | 1 |
| 10% Corn tissue homogenate | 1 |
| 10% Sweet potato tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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