Starch Branching Enzyme (SBE) Activity Colorimetric Assay Kit (E-BC-K1204-M)
For research use only.
| Detection Principle | Starch Branching Enzyme (SBE) catalyzes the conversion of Amylose to Amylopectin. SBE can reduce the content of amylose and thus reduce the optical absorption value of starchiodine complex at 660 nm. The percentage of absorbance decline in a certain time can reflect the activity of SBE. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (650-670 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.5 U/g wet tissue |
| Detection Range | 1.5-15.0 U/g wet tissue |
| Precision | inter-assay CV: 2.2-4.6% | intra-assay CV: 3.3-4.8% |
| Sample Volume | 200 μL(tissue homogenate) |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Corn tissue homogenate | 2-5 |
| 10% Red been tissue homogenate | 5-10 |
| 10% Peanut tissue homogenate | 5-15 |
| 10% Mung been tissue homogenate | 5-10 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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