HMGB-1 Polyclonal antibody (AN006010L)
For research use only.
| Verified Samples | Verified Samples in WB: HeLa, Jurkat, NIH/3T3, C6, PC-12 |
| Dilution | WB 1:500-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human HMGB-1 protein expressed by E.coli |
| Abbre | HMGB-1 |
| Synonyms | High mobility group protein 1, high mobility group protein B1, high-mobility group box 1, HMG1, HMG-1, HMG1DKFZp686A04236, HMG3, HMGB1, SBP-1, Chromosomal protein, DKFZp686A04236, High mobility group 1, High mobility group box 1, high-mobility group (nonhistone chromosomal) protein 1, HMGB 1, nonhistone, NONHISTONE CHROMOSOMAL PROTEIN HMG1, SBP 1, Sulfoglucuronyl carbohydrate binding protein, SBP, HMGB, High Mobility Group Protein, High mobility group protein B, HMG, HMG-1, HMG1, HMG3, SBP-1, HMGB1, Amphoterin, High Mobility Group Protein 1, High mobility group protein B1, High mobility group protein 1 (HMG-1) |
| Swissprot | |
| Calculated MW | 24 kDa |
| Observed MW |
29 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Chromosome, Cytoplasm, Endoplasmic reticulum, Endosome, Membrane, Nucleus, Secreted |
| Tissue Specificity | Ubiquitous. Expressed in platelets. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Epigenetics and Nuclear Signaling |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu . Residues 27‑43 and 178‑184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. |
Other Clones
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Unconjugated
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