HMGB-1 Polyclonal Antibody(Capture/Detector) (AN000120P)

For research use only.
Verified Samples |
Verified Samples in WB: Hela, Jurkat, A431, NIH/3T3, C6 Verified Samples in ELISA: Recombinant Human HMGB-1 protein, Human serum, Human plasma |
Dilution | WB 1:500-1:1000, ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL |
Isotype | Rabbit IgG |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Applications | WB, ELISA Capture/Detector |
Clonality | Polyclonal |
Immunogen | Recombinant Human HMGB-1 protein expressed by E.coli |
Abbre | HMGB-1 |
Synonyms | HMGB1, HMG1, High mobiLity group protein B1, High mobiLity group protein 1 (HMG-1) |
Swissprot | |
Calculated MW | 29 kDa |
Observed MW |
29 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Concentration | 1 mg/mL |
Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
Purification Method | Antigen Affinity Purification |
Research Areas | Epigenetics and Nuclear Signaling |
Conjugation | Unconjugated |
Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
background | Human High-mobility group box 1 protein (HMGB1),previously known as HMG-1 or amphoterin,is a member of the high mobility group box family of non-histone chromosomal proteins.HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly,both as an inflammatory mediator that promotes monocyte migration and cytokine secretion,and as a mediator of T cell-dendritic cell interaction. The cytokine activity of HBMG1 is restricted to the HMG B box,while the A box is associated with the helix-loop-helix domain of transcription factors. HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence,it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted,HMGB1 transduces cellular signals through its high affinity receptor,RAGE and,possibly,TlR2 and TlR4. Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse,rat,bovine and porcine HMGB1,respectively. |
Other Clones
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Unconjugated
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