For research use only.
Verified Samples |
Verified Samples in WB: 231 Verified Samples in IHC: Human brain, Human colorectal cancer |
Dilution | WB 1:500-1:2000, IHC 1:40-1:200 |
Clonality | Polyclonal |
Immunogen | Synthetic peptide of human HTRA1 |
Abbre | HTRA1 |
Synonyms | ARMD7, CARASIL, HTRA1, High-temperature requirement A serine peptidase 1, HtrA, HtrA serine peptidase 1, IGFBP5 protease, L56, ORF480, PRSS11 , Protease serine 11 (IGF binding), Serine protease 11, Serine protease HTRA1, Serine protease HTRA1 , protease serine 11 |
Swissprot | |
Calculated MW | 51 kDa |
Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Secreted. |
Concentration | 0.9 mg/mL |
Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
Purification Method | Antigen affinity purification |
Research Areas | Cancer, Signal transduction |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
background | This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. |
Other Clones
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