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Human DPD(Deoxypyridinoline) ELISA Kit (E-EL-H2020)

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All Size Price Qty
96T $ 609.00
48T $ 487.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 1.88 ng/mL
Detection Range 3.13-200 ng/mL
Sample Volume 50 μL
Total Assay Time 2 h 30 min
Reacitivity Human
Specificity This kit recognizes Human DPD in samples.No significant cross-reactivity or interference between Human DPD and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Competitive
Assay Type Competitive-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human DPD. During the reaction, Human DPD in the sample or standard competes with a fixed amount of Human DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human DPD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human DPD in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Dihydropyrimidine dehydrogenase (DPD) is an enzyme that is primarily involved in human pyrimidine metabolism and has the same metabolic effects on the pyrimidine analogue 5-FU (5-Fluorouracil, 5-fluorouracil). DPD is the initiator and rate-limiting enzyme of 5-FU metabolism in vivo, and 80%-90% of 5-FU is excreted from the liver after DPD metabolism to dihydrofluorouracil. DPD is closely related to the efficacy, metabolic efficiency and toxicity of 5-FU. If there is a DPD enzyme defect in the body, it may lead to the accumulation of 5-FU in the body, and eventually cause adverse consequences due to drug toxicity.
Research Area Metabolism
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