Human G6PD (Glucose 6 Phosphate Dehydrogenase) CLIA Kit (E-CL-H1125)

Name: Human G6PD (Glucose 6 Phosphate Dehydrogenase) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1125
Price: 180.0 USD
Availability: InStock

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Human G6PD (Glucose 6 Phosphate Dehydrogenase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human G6PD in samples.No significant cross-reactivity or interference between Human G6PD and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	56354.000	64128.000	60241.000	60212
2000	28004.000	30578.000	29291.000	29262
1000	13591.000	13501.000	13546.000	13517
500	6788.000	7284.000	7036.000	7007
250	3705.000	3371.000	3538.000	3509
125	1806.000	1594.000	1700.000	1671
62.5	759.000	817.000	788.000	759
0	29.000	29.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	206.490	361.060	1587.890	209.520	373.100	1659.470
Standard deviation	20.190	38.530	140.690	26.970	32.680	124.130
CV（%）	9.780	10.670	8.860	12.870	8.760	7.480

Recovery

The recovery of Human G6PD was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-104	95
EDTA plasma （n=8）	97-109	103
Cell culture media （n=8）	96-110	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human G6PD and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-99	95-112	95-111
1:2	Average	91	103	102
1:4	Range	99-116	101-114	89-102
1:4	Average	107	107	96
1:8	Range	102-115	88-104	88-101
1:8	Average	109	95	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human G6PD. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human G6PD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human G6PD, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human G6PD. You can calculate the concentration of Human G6PD in the samples by comparing the RLU value of the samples to the standard curve.

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