Struggling with the details of your ELISA experiments? Inaccurate pipetting, incomplete washing, inconsistent incubation times; even a tiny mistake can ruin your results! Don’t worry! We’ve prepared the ultimate ELISA guide for you. It not only breaks down the core steps but also brings together operation videos for all Elabscience® ELISA series, helping you avoid common pitfalls and master ELISA with ease!
Table of Contents
1. Master the Core Four Steps to Nail Most ELISAs
2. Explore the Elabscience® ELISA Portfolio: Find Your Perfect Fit!
3. Detailed Operation Videos for Each Series: Point-by-Point Guidance!
01 Master the Core Four Steps to Nail Most ELISAs
No matter which type of ELISA kit you use, the core principles and workflow are similar. Mastering the following key points is essential for obtaining reliable data.
1. Preparation and Sample Addition
Reagents to Room Temperature: Always bring all reagents to room temperature before use.
Standards are Key: Reconstitute and dilute standards exactly as instructed. Always change pipette tips between dilutions.
Slow Addition Along the Well Wall: Add samples slowly along the bottom of the well wall to avoid bubbles and splashing.
Cover and Incubate: Seal the plate with sealing film and incubate for the specified time at the required temperature.
Wash Thoroughly: For manual washing, we recommend: fill wells completely with wash buffer, let stand for 60 seconds, aspirate and flick, and repeat 3 times. After the final wash, blot the plate firmly onto absorbent paper to remove residual liquid.
3. Color Development and Stop
Develop in the Dark: Add TMB substrate and develop protected from light. Stop the reaction once a clear blue gradient is visible.
Stop Promptly: Adding the stop solution should immediately change the color from blue to yellow. Read the plate promptly afterward.
02 Explore the Elabscience® ELISA Portfolio: Find Your Perfect Fit!
The versatile, reliable choice for routine protein detection, offering stable and reproducible results across nearly a thousand targets.
Streamline your workflow. Complete your assay in just 1.5 hours without compromising specificity, ideal for rapid screening.
Conserve precious samples with a 25 μL sample volume, while maintaining performance comparable to traditional methods.
Detect low-abundance targets with fg/mL level sensitivity, powered by an optimized signal amplification system.
Specifically optimized for cell culture samples and include specific quality controls to ensure accurate results from cell culture supernatants or lysates.
A cost-effective and flexible option for researchers who require custom assay development.
Analyze cytokine secretion at the single-cell level, a gold standard for immunology research.
03 Detailed Operation Videos for Each Series: Point-by-Point Guidance!
Explore our detailed video guides for each series to help you master the specific nuances and key steps beyond the basic protocol!
Sandwich ELISA Operation Guide Video
Competitive ELISA Operation Guide Video
QuicKey Pro ELISA (Sandwich Method) Operation Guide Video
QuicKey Pro ELISA (Competitive Method) Operation Guide Video
Uncoated ELISA Kit Operation Guide Video
If you have any questions about ELISA experiments or our products, feel free to message us on our official WeChat account or contact our technical support team!
More ELISA product operation videos are coming soon! Stay tuned by following us.
