For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, Hela, A549 |
| Dilution | WB 1:500-1:2000 |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of human IL6 |
| Abbre | IL6 |
| Synonyms | IFNB, Interleukin HP, Interferon Beta, B-Cell Stimulatory Factor, IFN-Beta, B-cell hybridoma growth factor, B-Cell Stimulatory Factor 2, BSF2, BSF-2, CDF, CTL Differentiation Factor, HGF, HSF, Hybridoma Growth Factor, IFN-Beta-2, IL-6, Interferon Beta-2, Interleukin 6, Interleukin HP-1, Interleukin-6, IL6, IFNB2, B cell differentiation factor, B cell stimulatory factor 2, BSF 2, Hepatocyte stimulatory factor, Hybridoma growth factor Interferon beta-2, IL 6, Interferon beta 2, Interleukin 6 (interferon beta 2), Interleukin BSF 2, Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1 |
| Swissprot | |
| Calculated MW | 24 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted. |
| Concentration | 1.1 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Metabolism, Immunology, Neuroscience |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells,T-cells,hepatocytes,hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine,a cytokine produced from muscle,and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally,IL-6 is involved in hematopoiesis,bone metabolism,and cancer progression,and has been defined an essential role in directing transition from innate to acquired immunity. |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
{{item.title}}
IF：{{item.impact}}
Journal：{{item.journal}}
DOI：{{item.doi}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
{{item.content}}
