Inhibition and Production of Superoxide Anionic Colorimetric Assay Kit(WST-1 Method) (E-BC-K001-M)
For research use only.
| Detection Principle | Superoxide anion free radicals are produced through the reaction system of xanthine and xanthine oxidase. WST-1 (a water-soluble tetrazolium salt) can react with the generated superoxide anion to produce water-soluble formazan. When the tested sample contains the superoxide anion free radical inhibitor, it can inhibit the formation of formazan. When the tested sample contains the substance that produces superoxide anion free radical, it can promote the formation of formazan dye. By colorimetric analysis of WST-1 products, the units of activity of inhibition or production of superoxide anion radical in samples can be calculated. |
| Sample Type | Serum,Plasma,Urine,Cell,Cell culture supernatant,leucocyte |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Oxidative Stress , Ferroptosis |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Precision | inter-assay CV: 5.8% | intra-assay CV: 0.02 |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 4-7 |
| Mouse serum | 15-25 |
| Rat serum | 25-35 |
| Human saliva | 1 |
| HepG2 culture supernatant | 1 |
| 10% Rat brain tissue homogenate | 150-200 |
| 10% Rat liver tissue homogenate | 500-600 |
| 10% Mouse liver tissue homogenate | 500-600 |
| 10% Mouse heart tissue homogenate | 150-200 |
| 10% Epipremnum aureum tissue homogenate | 20-30 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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