ITGAX Polyclonal Antibody (E-AB-70017)
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For research use only.
| Verified Samples |
Verified Samples in WB: RAW264.7, Mouse spleen, Mouse lung, Mouse thymus gland Verified Samples in IHC: Human amygdalitis, Rat inflammatory spleen, Human spleen Verified Samples in IF: Mouse inflammatory spleen |
| Dilution | WB 1:500-1:1000, IHC 1:100-1:300, IF 1:100-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse CD11c |
| Abbre | ITGAX |
| Synonyms | 95, 95 alpha chain, CD 11c, CD11 antigen like family member C, CD11 antigen-like family member C, CD11c, CD11c antigen, CR4, Complement component 3 receptor 4 subunit, Integrin aX, Integrin alpha X, Integrin alpha X chain, Integrin alpha-X, Integrin subunit alpha X, in |
| Swissprot | |
| Calculated MW | 120-150 kDa |
| Observed MW |
150 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane. |
| Concentration | 0.98 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Immunology, Signal Transduction, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a type I transmembrane protein present on a variety of cells,including monocytes/macrophages,granulocytes,NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis. |
Other Clones
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Other Formats
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Unconjugated
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