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For research use only.

Verified Samples Verified Samples in WB: 293T, HepG2, HeLa
Verified Samples in IHC: Human gastric cancer, Human lung cancer, Human colon carcinoma
Dilution WB 1:500-1:2000,  IHC 1:100-1:200
Isotype IgG
Host Rabbit
Reactivity Human
Applications WB,  IHC
Clonality Polyclonal
Immunogen Recombinant fusion protein of human HIRA (NP_003316.3).
Synonyms DGCR1,  HIRA,  TUP1,  TUPLE1
Swissprot
Calculated MW 89 kDa/111 kDa
Observed MW 112 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus, Nucleus, PML body, Primarily, though not exclusively, localized to the nucleus, Localizes to PML bodies immediately prior to onset of senescence.
Concentration 1 mg/mL
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Affinity purification
Research Areas Cancer,  Epigenetics and Nuclear Signaling,  Developmental Biology,  Stem Cells
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
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Unconjugated

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