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LTE4(Leukotriene E4) ELISA Kit (E-EL-0114)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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All Size Price Qty
96T $ 495.00
48T $ 396.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 18.75 pg/mL
Detection Range 31.25-2000 pg/mL
Sample Volume 50 μL
Total Assay Time 2 h 30 min
Reacitivity Universal
Specificity This kit recognizes Universal LTE4 in samples.No significant cross-reactivity or interference between Universal LTE4 and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Competitive
Assay Type Competitive-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal LTE4. During the reaction, Universal LTE4 in the sample or standard competes with a fixed amount of Universal LTE4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal LTE4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal LTE4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Leukotriene E4 is a cysteinyl-leukotriene substance involved in the inflammatory response and is produced by several types of white blood cells, including eosinophils, mast cells, macrophages, and basophils, and recently neutrophils with platelet adhesion have been found to produce leukotriene E4.
Research Area Immunology
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