Mini Sample Rat MIP-1α (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit (E-MSEL-R0004)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MIP-1α . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MIP-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MIP-1α , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 53 nm. The OD value is proportional to the concentration of MIP-1α . You can calculate the concentration of MIP-1α in the samples by comparing the OD of the samples to the standard curve.

CCL3, also known as macrophage inflammatory protein 1α (MIP-1α) and LD78, is a member of the chemokine beta or CC subfamily and is closely related to CCL4/ MIP-1β. Chemokines include a large family of small secreted proteins involved in immune and inflammatory responses. CCL3 can be expressed in a variety of hematopoietic cells, fibroblasts, smooth muscle cells and epithelial cells. The amino acid sequence of CCL3 in mature rats showed 74%, 91% and 88% homology with that of human, mouse and cotton rats, respectively. CCL3 is a chemokine of about 8 kDa that forms complexes with sulfated proteoglycans. In a reversible process, CCL3 binds to non-covalently linked dimers, which then form tetramers and high molecular weight polymers. These complexes of CCL3 are not protected from proteolytic digestion by insulin-degrading enzymes (ides), which can cut monomer chemokines (6). CCL3 performs its biological functions through interactions with CCR1, CCR3, and CCR5. By inducing the internalization of the chemokine receptor D6, CCL3 is cleared from the extracellular space. And cell activation of many hematopoietic cell types, including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils. CCL3 is also known as a stem cell inhibitor (SCI), which inhibits the proliferation of hematopoietic progenitor cells (3). The biological activity of CCL3 contributes to tumor metastasis and the inflammatory components of viral infections, rheumatoid arthritis, and hepatitis, although it can also inhibit HIV replication. CCL3 also promotes hyperalgesia by sensitizing sensory neurons to trpv1 mediated harmful stimuli.