Product Summary

Sensitivity	0.47 ng/mL
Detection Range	0.78-50 ng/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Mouse
Specificity	This kit recognizes MPO in samples.No significant cross-reactivity or interference between MPO and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MPO . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MPO , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 8 nm. The OD value is proportional to the concentration of MPO . You can calculate the concentration of MPO in the samples by comparing the OD of the samples to the standard curve.

Background

Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Mouse MPO shares 87% amino acid sequence identity with that of human MPO.

Gene Alias	Mpo
Gene ID	17523
Uniport ID	P11247
Protein Alias	Mpo
Research Area	Immunology , Cardiovascular , Cancer , Metabolism

Mouse MPO (Myeloperoxidase) ELISA Kit - procedures

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Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Mouse MPO (Myeloperoxidase) ELISA Kit (E-EL-M3125)

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