NADP-Isocitrate Dehydrogenase (NADP-IDH) Activity Assay Kit (E-BC-K554-M)
For research use only.
| Detection Principle | Isocitrate dehydrogenase converts isocitrate to α- ketoglutaric acid, while converting NADP+ to NADPH. Under the action of electron coupling agents, the generated NADPH has a characteristic absorption peak at 450 nm. The activity of NADP-IDH can be calculated by measuring the change of absorbance value at 450 nm. |
| Sample Type | Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Other Reagents Required | Double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.10 U/L |
| Detection Range | 0.10-50.47 U/L |
| Precision | inter-assay CV: 4.3-11.4% | intra-assay CV: 5.0-6.0% |
| Sample Volume | 20 μL(tissue/cell homogenate) |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat kidney tissue homogenate | 10-60 |
| 10% Rat brain tissue homogenate | 5-10 |
| 10% Rat liver tissue homogenate | 10-80 |
| 1×10^6 CHO cells | 1 |
| 1 × 10^6 RAW cells | 1 |
| 1 × 10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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